Research Article
An Efficient Protocol for Deriving Liver Stem Cells from Neonatal Mice: Validating Its Differentiation Potential
Table 1
List of primers used in gene expression profiling.
| | Genes | Primer sequence (5′-3′) | Tm | Length |
| | HNF-1α | FP: 5′-CGAAGATGGTCAAGTCGTAC-3′
RP: 5′-GGCAAACCAGTTGTAGACAC-3′ | 55°C | 500 bp |
| | HNF-1β | FP: 5′-TTCAGTCAACAGAACCAGGG-3′
RP: 5′-CTCTGTGCAATGGCCATGAC-3′ | 57.2°C | 721 bp |
| | HNF-3α | FP: 5′-CATGAGAGCAACGACTGGAA-3′
RP: 5′-TTGGCGTAGGACATGTTGAA-3′ | 55.2°C | 182 bp |
| | HNF-3β | FP: 5′-AGAGGACTGAGGTAACTGAC-3′
RP: 5′-GACTCGGACTCAGGTGAGGT-3′ | 60.2°C | 415 bp |
| | HNF-6 | FP: 5′-CAGCGTATCACCACCGAGCT-5′
RP: 5′-CTCTGTCCTTCCCATGTTCT-3′ | 55°C | 250 bp |
| |
C/EBP-α | FP: 5′-TGGACAAGAACAGCAACGAG-3′
RP: 5′-TCACTGGTCAACTCCAGCAC-3′ | 56°C | 126 bp |
| |
C/EBP-β | FP: 5′-GAGCGACGAGTACAAGATGCG-3′
RP: 5′-TTGTGCTGCGTCTCCAGGTTG-3′ | 61°C | 95 bp |
| | GATA-4 | FP: 5′-CTGTCATCTCACTATGGGCA-3′
RP: 5′-CAAGTCCGAGCAGGAATTTG-3′ | 59°C | 257 bp |
| | CK8 | FP: 5′-ATCGAGATCACCACCTACCG-3′
RP: 5′-TGAAGCCAGGGCTAGTGAGT-3′ | 55°C | 127 bp |
| | CK18 | FP: 5′-CGAGGCACTCAAGGAAGAAC-3′
RP: 5′-GCTGAGGTCCTGAGATTTGG-3′ | 57°C | 130 bp |
| | CK19 | FP: 5′-ACCCTCCCGAGATTACAACC-3′
RP: 5′-CAAGGCGTGTTCTGTCTCAA-3′ | 58°C | 160 bp |
| | p450 Cyp3a11 | FP: 5′-TGAGGCAGAAGGCAAAGAAA-3′
RP: 5′-GGTATTCCATCTCCATCACA-3′ | 55°C | 590 bp |
| | PXMP1-L | FP: 5′-CTTCAGACCCAGAGAGAGCTG-3′
RP: 5′-CCCGTGTTGCCTGTGATGAGC-3′ | 62°C | 475 bp |
| | Albumin | FP: 5′-TGAACTGGCTGACTGCTGTG-3′
RP: 5′-CATCCTTGGCCTCAGCATAG-3′ | 57°C | 718 bp |
| | Transthyretin | FP: 5′-AGTCCTGGATGCTGTCCGAG-3′
RP: 5′-TTCCTGAGCTGCTAACACGG-3′ | 62°C | 440 bp |
| | AFP | FP: 5′-TCGTATTCCAACAGGAGG-3′
RP: 5′-AGGCTTTTGCTTCACCAG-3′ | 55°C | 174 bp |
| | AAT | FP: 5′-AATGGAAGAAGCCATTCGAT-3′
RP: 5′-AAGACTGTAACTGCTGCAGC-3′ | 57.2°C | 484 bp |
| β-actin
(internal control) | FP: 5′-TTCCTTCTTGGGTATGGAAT-3′
RP: 5′-GAGCAATGATCTTGATCTTC-3′ | 55°C | 206 bp |
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