Quercetin Reduces Ehrlich Tumor-Induced Cancer Pain in Mice

Figure 8

Naloxone did not reverse the effect of quercetin in reducing Ehrlich tumor cells-induced neutrophil recruitment. Mice were treated with quercetin (100 mg/kg, i.p., starting 10 min after tumor administration) during 8 days after the injection of Ehrlich tumor cells (1 × 10⁶ or 1 × 10⁷ cells/25 μL) or saline and, in the 8th day, one group of mice that received quercetin was also treated with naloxone (1 mg/kg i.p. diluted in saline) or its vehicle 1 h before the treatment with quercetin. The neutrophil recruitment was evaluated in samples of paw skin collected after 3 h of the treatment with quercetin by the myeloperoxidase (MPO) activity assay. Data are presented as means ± SEM of six mice per group per experiment and representative of two separated experiments: compared to the saline group and compared to the tumor group. One-way ANOVA followed by Tukey’s test.