Immunosuppressive Drugs Affect High-Mannose/Hybrid N-Glycans on Human Allostimulated Leukocytes
Table 1
Cyclosporin A (CsA) and rapamycin- (Rapa-) affected GNA-positive glycoproteins identified by MS/MS. Proteins bearing high mannose/hybrid-type oligosaccharides expressed on MLR-activated leukocytes treated with a combination of CsA (150 ng per 1 mL medium) and Rapa (12 ng per 1 mL medium) were identified in MS/MS analysis by comparison of the results with the Sprot database. Ten glycoproteins with the highest protein score for each band are presented.
Band no.
Accession no.
Protein name
Mascot score
Number of significant peptide matches
Protein sequence coverage [%]
Mass based on amino acid sequence [kDa]
Relative mass calculated based on standard proteins [kDa]
P05106
Integrin beta-3
6117
145
34
90.2
P14625
Endoplasmin
692
27
23
92.6
P05556
Integrin beta-1
618
19
16
91.7
Q9NZ08
Endoplasmic reticulum aminopeptidase 1
321
8
8
100.0
P19367
Hexokinase-1
315
7
7
100.0
1
P11279
Lysosome-associated membrane glycoprotein 1
275
7
6
45.4
102.4
O00462
Beta-mannosidase
236
9
9
101.8
P12259
Coagulation factor V
226
6
6
252.7
Q99467
CD180 antigen
205
2
2
75.2
Q8TB96
T-cell immunomodulatory protein
124
3
3
68.5
P05164
Myeloperoxidase
2491
88
25
83.8
P07237
Protein disulfide isomerase
502
18
14
57.1
P51688
N-Sulphoglucosamine sulphohydrolase
420
13
10
56.7
P16278
Beta-galactosidase
519
9
9
76.0
2
P00488
Coagulation factor XIII A chain
581
14
14
83.2
60.5
P01876
Ig alpha-1 chain C region
337
8
7
37.6
Q9Y4L1
Hypoxia upregulated protein 1
319
11
9
111.3
Q9NZK5
Adenosine deaminase CECR1
234
11
10
59.0
P08236
Beta-glucuronidase
208
6
5
74.7
Q9UHL4
Dipeptidyl peptidase 2
206
5
5
54.3
Q6P4A8
Phospholipase B-like 1
596
18
8
63.2
P15586
N-Acetylglucosamine-6-sulfatase
287
9
5
62.0
O14773
Tripeptidyl-peptidase 1
250
6
4
61.2
P01889
HLA class I histocompatibility antigen, B-7 alpha chain
172
3
3
40.4
Q9BS26
Endoplasmic reticulum resident protein 44
166
5
5
46.9
3
Q07000
HLA class I histocompatibility antigen, Cw-15 alpha chain
121
3
3
40.8
42.6
P05121
Plasminogen activator inhibitor 1
84
4
4
45.0
P04233
HLA class II histocompatibility antigen gamma chain
76
1
1
33.5
P10314
HLA class I histocompatibility antigen, A-32 alpha chain
