Evaluation of the Selenotranscriptome Expression in Two Hepatocellular Carcinoma Cell Lines

Figure 1

Expression of selenoprotein genes analyzed by means of RT-qPCR. The average value for the expression of these genes was obtained from three independent experiments. (a) shows the downregulated genes in HepG2 and Huh7 versus normal hepatocytes (DIO1, DIO2, and SELO) while (b) shows the ten upregulated genes (GPX4, GPX7, SELK, SELM, SELN, SELT, SELV, SEP15, SEPW1, and TrxR1). In each reaction, the expression levels were normalized to the average of the control gene (18S rRNA) and expressed as arbitrary units. The mRNA levels in hepatocytes, HepG2, and Huh7 were evaluated by using the ΔΔCt method. Significant differences in relative gene expression between hepatocytes and HepG2 or Huh7 are marked by ( value < 0.05) and ( value < 0.01).

(a)

(b)