Research Article
Ex Vivo Nicotine Stimulation Augments the Efficacy of Human Peripheral Blood Mononuclear Cell-Derived Dendritic Cell Vaccination via Activating Akt-S6 Pathway
Figure 6
Nicotine increases surface molecule expression in human semimature DCs by activating Akt-S6 pathway. (a–e) DCs derived from human PBMC with human GM-CSF and IL-4 were conferred with LY294002 (10 μmol/L) or Wortmannin (10 μmol/L) 2 h prior to 12~15 h nicotine (10−7 mol/L) stimulation. The effect of Akt inhibition on nicotine-increased expression of CD80 (a), MHC class I (b), MHC class II (c), CD86 (d), and 4-1BBL (e) was determined by flow cytometry. Numbers in histogram indicated mean fluorescence intensity (MFI) of test samples. Data were given as mean ± SEM, one-way ANOVA with post Newman-Keuls test. , , and . One representative from 3 independent experiments is shown. Ni: nicotine; LY: LY294002; WM (Wort): Wortmannin.
| (a) |
| (b) |
| (c) |
| (d) |
| (e) |