Polymorphisms of Homologous Recombination RAD51, RAD51B, XRCC2, and XRCC3 Genes and the Risk of Prostate Cancer

Table 3

Details of PCR-RFLP of the studied SNPs.

SNP	Primer sequences	Annealing temp. (°C)	Product size (bp)	Enzyme	Genotype	Fragment sizes (bp)
rs1801320	(F) 5′TGGGAACTGCAACTCATCTGG3′; (R) 5′GCGCTCCTCTCTCCAGCAG3′				GG	71, 86
		65	157	MvaI	GC	71, 86, 157
					CC	157
rs3218536	(F) 5′CGTCAATGGAGGAGAAAGTGTG3′; (R) 5′TCGAGAGGCATGAGAAGGTT3′				GG	166
		64	166	SexA1	GA	79, 87, 166
					AA	79, 87
rs861539	(F) 5′TAAGAAGGTCCCCGTACTCC3′; (R) 5′CTGCGCATCAACCAGGTGAG3′				CC	34, 161
		64	195	NlaIII	CT	56, 105, 161
					TT	56, 105