Transcription Factor HBP1 Enhances Radiosensitivity by Inducing Apoptosis in Prostate Cancer Cell Lines

Figure 2

The establishment of prostate cancer cell lines with different expression levels of HBP1 gene. (a) Western blot assay was used to detect the protein level of HBP1 in monoclonal cell lines stably transfected by pBaBE-vector (Vector) and pBaBE-HBP1 (HBP1 OE) plasmid in DU-145 cells. (b) The mRNA level of the HBP1 downstream genes was detected via RT-qPCR including CCND, MIF, and N-Myc in Vector and HBP1 OE group. versus control. (c) Western blot assay was used to detect the protein level of HBP1 in monoclonal cell lines stably transfected by pGenesil-scramble (Con shRNA) and pGenesil-HBP1-shRNA (HBP1 shRNA) plasmid in DU-145 cells. (d) The mRNA level of the HBP1 downstream genes was detected via RT-qPCR including CCND, MIF, and N-Myc in Con shRNA and HBP1 shRNA groups. versus control.

(a)

(b)

(c)

(d)