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Analytical Cellular Pathology
Volume 2016, Article ID 7486989, 8 pages
Research Article

Comparison between Manual and Automated Methods for Ki-67 Immunoexpression Quantification in Ameloblastomas

1Department of Research, School of Dentistry, Universidad Juárez del Estado de Durango, Durango, DGO, Mexico
2Department of Health Care, Universidad Autónoma Metropolitana, Unidad Xochimilco, Mexico City, Mexico
3Microbiology and Pathology Department, Dentistry Research Institute, Health Sciences University Center, Universidad de Guadalajara, Guadalajara, JAL, Mexico
4School of Health Sciences, Universidad Anahuac Norte, Lomas Anáhuac, Huixquilucan, MEX, Mexico
5Department of Molecular Pathology, School of Dentistry, Universidad de la República, Uruguay, Montevideo, Uruguay

Received 5 August 2016; Accepted 27 September 2016

Academic Editor: Giovanni Tuccari

Copyright © 2016 Rogelio González-González et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Ameloblastoma is a common and unpredictable odontogenic tumor with high relapse rates. Several studies assessing the proliferative capacity of these neoplasms have been published, mainly using the protein Ki-67. Cell counts must be completed to determine the cell proliferation rate. Multiple methods have been developed for this purpose. The most widely used method is the labeling index, which has undergone changes over time to better facilitate cell counting. Here, we compared manual cell counting methods with automated cell counting (ImmunoRatio) to determine the relative effectiveness of these methods. The results suggest that ImmunoRatio, a free software tool, may be highly advantageous and provide results similar to manual cell counting methods when used with the appropriate calibration. However, ImmunoRatio has flaws that may affect the labeling index results. Therefore, this automated cell counting method must be supplemented with manual cell counting methods.