Procedures of single-cell sequencing (SCS) technology in cancer treatment. (1, 2) The patient’s sample is collected and then the single cell is isolated from the sample by means of serial dilution, mouth pipetting, flow sorting, robotic micromanipulation, and/or microfluidic platforms. (3) The nucleotide of the single cell is isolated and amplified by specific methods such as whole-genome amplification (WGA), whole-transcriptome amplification (WTA), and ChIP precipitation, which permit analysis at the genomic, transcriptomic, and epigenomic level. (4, 5) The amplified nucleotide is sequenced by a gene sequencer and the information is analyzed using bioinformatic methods. (6) The “omics” data can aid the clinician to determine an optimized treatment strategy.