Figure 1: Study flowchart. SD rat models with myocardial cell injury (male, 8 weeks, weight 200 g~250 g) were divided into 7 groups: sham-operated group (SG), limb ischemia reperfusion group (IRG), limb ischemia reperfusion-preconditioning group (IRPG), sevoflurane-treated group (SevG), nicotinamide inhibitor-treated group (NG), 3-MA inhibitor-treated group (MG), and NAM+3-MA inhibitor-treated group (NMG).