Aloesin Suppresses Cell Growth and Metastasis in Ovarian Cancer SKOV3 Cells through the Inhibition of the MAPK Signaling Pathway

Figure 3

Aloesin inhibits cell migration and invasion in SKOV3 cells. (a) Wound healing assay was performed to observe the effects of aloesin treatment on SKOV3 cell wound recovery ability. (b) Quantification of the healed wound area. (c) Transwell migration and invasion assays were performed to observe the effects of aloesin treatment on SKOV3 cell migration and invasion abilities. For each group of cells, five fields were randomly photographed and representative images were shown here. (d) In the transwell migration and invasion assays, transmigrated cells were counted from the photographed 5 fields in each group. The average cell numbers were calculated in each group. (e) Western blot analysis of major matrix metalloproteinase MMP-2 and MMP-9 expression in SKOV3 cells with distinct doses of aloesin. ; ; . (f) Representative photos of histological H&E staining of lung metastasis were shown for each group. (g) A bar graph summarizes the number of lung metastasis node in the two groups. (h) A bar graph summarizes the incidence of lung metastasis in the two groups.

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