Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 Cells

Figure 4

SSP-dependent BrdU incorporation in A549 cells. Cells were seeded in 24-well plates at high density and cultivated for further 3 days in the absence or presence of 50 nM SSP. Cells were then pulsed for 4 h with BrdU. BrdU incorporation was visualized with anti-BrdU antibodies and secondary fluorescently labelled antibodies. Fluorescence and corresponding phase micrographs are shown.