Metformin Treatment Inhibits Motility and Invasion of Glioblastoma Cancer Cells

Figure 2

Metformin treatment inhibits cell motility in SF268 and U87 cells. (a) Control and metformin-treated SF268 cells were allowed to form monolayers before wounding with a micropipette. The same frame was imaged directly (upper micrographs) or 24 h (lower micrographs) after wound. (b) Mobility in (a) was quantified by measuring the width of single wound at 12 distinct points. The average rate of wound closure was calculated in μm/hr. Data are the mean ± SEM from 3 different wound healing experiments. indicates that the values are significant with . (c) SF268 cells were treated with metformin (2.5 mM) (Supplemental movie S2) for 24 h or left untreated (Supplemental movie S1). Quantitation of their net path is expressed in μm. indicates that the values are significant with . U87 cells were treated with metformin (2.5 mM) (Supplemental movie S4) for 24 h or left untreated (Supplemental movie S3). Quantitation of their net path is expressed in μm. indicates that the values are significant with .

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