Heparan Sulfate Proteoglycans in Human Colorectal Cancer

Figure 2

Expression of syndecan-2 (Syn-2) in normal colorectal cell line (CCD 841 CoN), in nonmetastatic CRC cell line CACO-2, and in high metastatic CRC cell line HCT-116. Immunostaining (red) was detected using an antibody specific for syndecan-2 (Santa Cruz) and an Alexa Fluor 594-labeled secondary antibody. Cell nuclei were stained with DAPI (blue). Images were obtained using a confocal a microscope Leica Microsystems TCS SP8 and analyzed by software LAS-AF.