LncRNA LOXL1-AS1 Promotes the Proliferation and Metastasis of Medulloblastoma by Activating the PI3K/AKT Pathway

Figure 5

Depletion of LOXL1-AS1 inhibited tumor growth in medulloblastoma in vivo. (a) qRT-PCR analysis of relative LOXL1-AS1 levels in D283 cells to confirm the knockdown efficacy of shRNA1 (the first synthesized shRNA against LOXL1-AS1). (b) D283 cells were pretreated with the scramble (control group) or shRNA1 prior to inoculation into mice. Four weeks after inoculation, neoplasia were resected and weighed. (c) During the 4-week monitoring, tumor dimensions were measured and tumor volume was calculated for each group of mice. (d) The neoplasia from control mice or shRNA1-treated mice were subject to immunohistochemistry analysis of Ki-67 (an indicator of cell proliferation) or TUNEL staining (indicating cell apoptosis). (e) The Ki-67-positive cells were calculated for control and shRNA1-treated mice. (f) TUNEL-positive cells were also quantified to show tumor cell apoptosis. ; .

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