(a)
(b)
(c)
Figure 1: Phenotype of circulating tumor cells isolated using ScreenCell Cyto kit. In representative images, nuclei are displayed in blue. Pseudocolors were changed for each of the four channels to optimize the visualization of the different signals. (a) Immunofluorescence for CD34, EpCAM, and CD45. Clusters of CD45- cells coexpressing EpCAM and CD34 are displayed. (b) and (c) Immunofluorescence for EpCAM, cytokeratins (CK) 8, 18, and 19, and CD45. Single CD45-/CK- cells expressing EpCAM (b). Cluster of CD45- cells coexpressing EpCAM and CK (c).