Figure 1: ILF2 was identified to directly interact with CREB. (a) MS analysis of CREB-binding proteins. The proteins immunoprecipitated by anti-CREB antibodies were analyzed by ESI-Q-TOF-MS in Bel-7402 cells for three independent times. (b) Endogenous CREB was immunoprecipitated by anti-CREB antibodies in Bel-7402 and SMMC-7721 cells, and the indicated proteins were measured by WB. The negative control for IP was immunoglobulin G (IgG). (c, d) The colocalization of CREB and other transcription factors in Bel-7402 and SMMC-7721. The nuclei were stained with DAPI. Representative images of three independent experiments were shown. Cells were obtained for IF by anti-CREB and corresponding antibodies. Scale bar, 15 μm. (e, f) The interactions between CREB and ILF2 or ILF3 were analyzed by PLA. The rightmost graphic was the enlargements of the boxes. Scale bar, 50 μm.