A Hopeful Natural Product, Pristimerin, Induces Apoptosis, Cell Cycle Arrest, and Autophagy in Esophageal Cancer Cells

Figure 1

Pristimerin inhibits human esophageal cancer cell proliferation. (a) Molecular formula of pristimerin. (b) Eca109 and Ec9706 cells were treated with pristimerin at concentrations of 0.5, 1.0, 1.5, 2.0, and 4.0 μmol·L^–1 for 48 h. Relative cell viability determined by the CCK-8 assay. (c) Cytometric analysis of Eca109 and Ec9706 cells treated with pristimerin (0.5, 1.0, 1.5, 2.0, and 4.0 μmol·L^–1, 48 hours). (d) Protein level of Bcl-2, Bax, caspase-3, and caspase-9. (e) Effect of pristimerin on the growth of Eca109 cells injected into nude mice. Male BALB/c nude mice were subcutaneously injected with Eca109 cells infected with pristimerin. Tumor volume and weight were monitored over time as indicated, and the tumor was excised and weighed after 21 days. versus control group. independent experiments for each group.

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