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Analytical Cellular Pathology
Volume 2019, Article ID 9365654, 7 pages
Research Article

HPV E6/E7, hTERT, and Ki67 mRNA RT-qPCR Assay for Detecting High-Grade Cervical Lesion with Microscope Slides

1Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Gangwon Province, Republic of Korea
2Department of Pathology, Yonsei University Wonju College of Medicine, Wonju, Gangwon Province, Republic of Korea
3Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan, Republic of Korea
4Department of Clinical Laboratory Science, Hyejeon College, Hongseong, Chungnam Province, Republic of Korea
5M&D, Inc., Wonju Eco Environmental Technology Center, Wonju, Gangwon Province, Republic of Korea

Correspondence should be addressed to Kwang Hwa Park; and Hyeyoung Lee;

Geehyuk Kim and Jemberu Taye contributed equally to this work.

Received 15 June 2018; Revised 2 November 2018; Accepted 4 November 2018; Published 14 January 2019

Academic Editor: Madhyastha Harishkumar

Copyright © 2019 Geehyuk Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


After breast and colon cancer, cervical cancer is the third most common cancer of women worldwide. Since human papillomavirus (HPV) infection is known to be the predominant cause of cervical cancer, molecular HPV screening is currently used along with cytological and histological examination methods for precancer diagnosis. Nevertheless, the sensitivity of the current HPV test is less than 80%; thus, many cervical cancer cases are not able to be diagnosed by HPV screening alone, and likewise, patients with cervical cancer are often determined to be HPV-negative by the current screening methods. Therefore, human telomerase reverse transcriptase (hTERT) and Ki67 previously identified as cancer markers were attempted. And cervical exfoliated cells of high-grade squamous intraepithelial lesion (HSIL), the most severe precancerous lesion of cancer, were used in the study. However, it takes a long time to collect enough specimens to conduct statistical analysis. Therefore, in the present study, microscope slides, cervical exfoliated cells on glass slides, were attempted. The results of the analysis demonstrated that hTERT and Ki67 expression levels were useful in distinguishing between cancerous and normal specimens, exhibiting a higher sensitivity and specificity than conventional HPV E6/E7 testing. And the study suggests clinical slide cell samples could be effectively used in the context of retrospective studies to identify novel biomarkers.