Figure 2: Analysis of the relative expression of hTERT (a) and Ki67 (b) in high-grade squamous intraepithelial lesion (HSIL) versus normal clinical samples using the delta Ct method. As shown in (a) and (b), normal and HSIL groups were distinguished by hTERT and Ki67 gene expression showing a statistically significant difference (), respectively. The cut-off value for distinguishing between positive and negative results is determined from the receiver operating characteristic (ROC) curve.