MicroRNA-342 Promotes the Malignant-Like Phenotype of Endometrial Stromal Cells via Regulation of Annexin A2

Figure 4

miR-342 enhances the malignant-like phenotype of endometriosis by targeting ANXA2. The endometrial stromal cells were transfected with NC mimic + NC vector, miR-342 mimic + NC vector, NC mimic + ANXA2 vector, or miR-342 mimic + ANXA2 vector. (a) The expression of miR-342 and ANXA2 determined using RT-qPCR. (b) Cell proliferation determined using CCK-8. (c) Cell migration assessed using Transwell migration assay. (d) Cell invasion assessed using Transwell invasion assay. (e) Cell apoptosis determined using flow cytometry. vs. NC mimic + NC vector; ^#vs. miR-342 mimic + NC vector. All data, representative of three independent experiments in triplicate, are shown as . Unpaired -test was adopted to analyze the differences between two experimental groups. Two-way ANOVA, followed by Tukey’s multiple comparison test, was performed to test data among multiple groups. RT-qPCR: reverse transcription quantitative polymerase chain reaction; miR: microRNA; ANXA2: Annexin A2; CCK8: cell counting kit-8; NC: negative control; ANOVA: analysis of variance.

(a)

(b)

(c)

(d)

(e)