Protective Effects of Astragalus Polysaccharide on Sepsis-Induced Acute Kidney Injury

Figure 3

LPS induced HK-2 cell damage. (a) CCK-8 assays were used to detect cell viability; (b) flow cytometry was used for assessment of cell apoptosis; (c) ELISA was performed for estimation of TNF-α, IL-1β, IL-6, and IL-8 expression and secretion. (d) Western blot was performed to determine the protein levels of caspase-3, caspase-9, Bax, Bcl-2, CHOP, GRP78, E-cadherin, α-SMA, Snail, and Vimentin, where GAPDH was used as an internal control; (e) RT-qPCR assay was used for evaluation of the mRNA levels of caspase-3, caspase-9, Bax, Bcl-2, CHOP, GRP78, E-cadherin, α-SMA, Snail, and Vimentin, in which GAPDH was used as an internal control gene to calculate the relative expression of the target genes. , , and versus the control group.

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