Protective Effects of Astragalus Polysaccharide on Sepsis-Induced Acute Kidney Injury

Figure 8

APS inhibited LPS-induced EMT in HK-2 cells. (a) Morphological changes of cells observed by light microscopy; (b) wound healing assay was used to detect cell migration; (c) Western blot was performed to detect the protein levels of E-cadherin, α-SMA, Snail, and Vimentin, and GAPDH was used to be an internal control; (d) RT-qPCR assay was used for evaluation of the mRNA levels of E-cadherin, α-SMA, Snail, and Vimentin, and GAPDH was used as an internal control gene to calculate the relative expression of the target genes. Compared with the control group, , , and ; compared with the LPS group, ^#, ^##, and ^###.

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