A task force of experts in the field of diagnostic DNA image cytometry, invited by the ESACP, and further scientists or physicians revealing experience in that diagnostic procedure (names are given in Addendum A), agreed upon the following 4th updated Consensus Report on Standardised Diagnostic DNA Image Cytometry during the 7th International Congress of that society in Caen, 2001. This report is based on the three preceding ones [6,14,17]. It deals with the following items: &#x2013; Critical review and update of the definitions given in the 1997 Consensus Update; &#x2013; Review and detailed description of basic terms, principles and algorithms for diagnostic interpretation; &#x2013; Recommendations concerning diagnostic or prognostic applications in specific fields of tumour pathology. This update is not aimed to substitute the 1997 consensus, but to make necessary addenda and give more detailed descriptions of those items not unequivocally to interpret by potential users of the methodology.

G. Haroske

J. P. A. Baak

H. Danielsen

F. Giroud

A. Gschwendtner

M. Oberholzer

A. Reith

P. Spieler

A. B&#xf6;cking

Fourth Updated ESACP Consensus Report on Diagnostic DNA Image Cytometry

In order to study the prognostic value of quantifying the chromatin structure of cell nuclei from patients with early ovarian cancer, low dimensionality adaptive fractal and Gray Level Cooccurrence Matrix texture feature vectors were extracted from nuclei images of monolayers and histological sections. Each light microscopy nucleus image was divided into a peripheral and a central part, representing 30&#x25; and 70&#x25; of the total area of the nucleus, respectively. Textural features were then extracted from the peripheral and central parts of the nuclei images. The adaptive feature extraction was based on Class Difference Matrices and Class Distance Matrices. These matrices were useful to illustrate the difference in chromatin texture between the good and bad prognosis classes of ovarian samples. Class Difference and Distance Matrices also clearly illustrated the difference in texture between the peripheral and central parts of cell nuclei. Both when working with nuclei images from monolayers and from histological sections it seems useful to extract separate features from the peripheral and central parts of the nuclei images.

Birgitte Nielsen

Fritz Albregtsen

Wanja Kildal

H&#xe5;vard E. Danielsen

Prognostic Classification of Early Ovarian Cancer Based on very Low Dimensionality Adaptive Texture Feature Vectors from Cell Nuclei from Monolayers and Histological Sections

Michael G. Ormerod

7th Congress of the European Society for Analytical Cellular Pathology, Caen, France, 1–5 April 2001

Fractal morphometry was used to investigate the ultrastructural features of the plasma membrane, perinuclear membrane and nuclear chromatin in SK&#x2010;BR&#x2010;3 human breast cancer cells undergoing apoptosis. Cells were incubated with 1 &#x3bc;M calcimycin (A23187) for 24 h. Cells in the early stage of apoptosis had fractal dimension (FD) values indicating that their plasma membranes were less rough (lower FD) than those of control cells, while their perinuclear membranes were unaffected. Changes of the chromatin texture within the entire nucleus and in selected nuclear domains were more pronounced in treated cells. This confirms that the morphological reorganization imputable to a loss of structural complexity (reduced FD) occurs in the early stage of apoptosis, is accompanied by the inhibition of distinct enzymatic events and precedes the onset of conventional cellular markers, which can only be detected during the active phases of the apoptotic process.

Christian Castelli

Gabriele A. Losa

Ultrastructural Complexity of Nuclear Components During Early Apoptotic Phases in Breast Cancer Cells

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Analytical Cellular Pathology provides a forum for pathologists and medical practitioners working in the cellular pathology field. Topics covered include cytology, carcinogenesis, cell receptors, biomarkers, diagnostic pathology, and immunopathology.

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Chief Editor&nbsp;Professor Dimitrios Karamichos&nbsp;focuses on investigating corneal wound healing and dystrophies with a particular interest in the effect of transforming growth factor-β3 or TGF- β3 on corneal stromal cells and their extracellular environment.

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Analytical Cellular Pathology - Table of contents 2001

title

Analytical Cellular Pathology provides a forum for pathologists and medical practitioners working in the cellular pathology field. Topics covered include cytology, carcinogenesis, cell receptors, biomarkers, diagnostic pathology, and immunopathology.

Analytical Cellular Pathology