Antigen-Experienced CD4lo T Cells Are Linked to Deficient Contraction of the Immune Response in Autoimmune Diabetes
Figure 5
The “CD4lo” T cells that persist among NOD Con A blasts are antigen-experienced. NOD spleen cells were cultured for 72 hrs with Con A, stained with anti-CD4-PE and anti-CD69-FITC (a), anti-CD62L-FITC (b), or anti-CD25-FITC (c), and analyzed by flow cytometry (20,000 events were collected for each analysis). The selected regions (boxes) in the dot plots mark the “CD4lo” population, and the numbers indicate their percentage in the total population. The underlined numbers indicated the percentage of CD4+ cells in the total population. (d) Freshly isolated NOD splenocytes were stained with anti-CD4-PE and IgG-FITC for comparison to baseline CD4 expression on naïve T cells. These data are representative of two experiments.
