Antigen-Experienced CD4lo T Cells Are Linked to Deficient Contraction of the Immune Response in Autoimmune Diabetes
Figure 7
Antigen-experienced “CD4lo” T cells give rise to “CD4hi” T cells. “CD4lo” T cells were enriched from the Con A blasts of NOD spleen cells as described in Figure 6. The “CD4lo” T cells or Con A blasts were cultured overnight in IL-2-containing complete medium, then each was labeled with CFSE before incubation on anti-CD3-coated plates. Twenty-four and 48 hrs later the cells were labeled with anti-CD4-PE and analyzed by flow cytometry to reveal cell divisions. Proliferating “CD4hi” T cells arising from the blasts are indicated in the encircled population the numbers indicate the percentage in the total cell population. For each analysis, 100,000 events were collected, and the results are representative of four experiments.
