Role of Structure-Based Changes due to Somatic Mutation in Highly Homologous DNA-Binding and DNA-Hydrolyzing Autoantibodies Exemplified by A23P Substitution in the VH Domain

<table>DNA hydrolysis by single-chain antibodies BV04-01, MRL-4 and their mutants. Supercoiled plasmid substrate was incubated overnight with 0.1 mkg of different ScFv in buffer, containing 20 mM Tris-HCl pH 7.5, 100 mM NaCl, and 1 mM MgCl<sub>2</sub>. Lane 1: control plasmid without an antibody; lane 2: plasmid with ScFv mutant BV04-01 VH A23P; lane 3: plasmid with ScFv mutant MRL-4 VH P23A; lane 4: plasmid with ScFv MRL-4; lane 5: plasmid with ScFv BV04-01.</table>

Autoimmune Diseases

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Figure 3

Figure 3: Role of Structure-Based Changes due to Somatic Mutation in Highly Homologous DNA-Binding and DNA-Hydrolyzing Autoantibodies Exemplified by A23P Substitution in the VH Domain 

Figure 3 | Role of Structure-Based Changes due to Somatic Mutation in Highly Homologous DNA-Binding and DNA-Hydrolyzing Autoantibodies Exemplified by A23P Substitution in the VH Domain 