A Novel Method for Real-Time, Continuous, Fluorescence-Based Analysis of Anti-DNA Abzyme Activity in Systemic Lupus

Table 1

Summarized comparison of DNAse I (from bovine pancreas, Ambion) and lupus anti-ssDNA antibody hydrolysis reactions.

Parameter/condition	DNAse I	Lupus anti-ssDNA antibody
pH	7.5	7.5
Active temperature range	+22–54°C	+4–37°C
Requirement for Ca^2+	—	0.5 mM
Requirement for Mg^2+	10 mM	5 mM
Time to reaction completion	30–40 min	8 hrs