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Autoimmune Diseases
Volume 2014 (2014), Article ID 237107, 8 pages
Research Article

Anti-Transglutaminase 6 Antibodies in Children and Young Adults with Cerebral Palsy

1Department of Pediatrics, Centre for Rehabilitation Research and Department of Clinical Medicine, School of Health and Medical Sciences, Örebro University, 70185 Örebro, Sweden
2Department of Neurology and Department of Neuroradiology, The Royal Hallamshire Hospital, Sheffield S10 2JF, UK
3Matrix Biology & Tissue Repair Research Unit, College of Biomedical and Life Sciences, School of Dentistry, Cardiff University, Cardiff CF14 4XY, UK

Received 18 November 2013; Revised 10 February 2014; Accepted 17 February 2014; Published 2 April 2014

Academic Editor: Kalle Kurppa

Copyright © 2014 Reidun Stenberg et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Sera were analysed for anti-TG6 antibodies using two different ELISA assays (Supplementary Figure). There was good agreement between these two methods in that 12/96 patients tested positive in the in house assay and 13/96 in a commercial assay with an overlap of 10 patients between the assays. We have also tested whether the results from the commercial assay would substantially alter the results with regards to the CP subgroups. The TP group remains the most prevalent in terms of TG6 antibody positivity, and the association is significant.

Supplementary Figure: Comparison of different ELISA methods for assessment of TG6 antibodies in serum. The in-house assay was performed as outlined in Materials and Methods and involved subtraction of the absorbance of a blank surface (no antigen) from that of a TG6-coated surface for calculation of relative antibody titres. The Zedira ELISA (IgA: 0312GE00; IgG 0412GE00) was carried out according to the manufacturer’s instructions and is based on absorbance measurement on antigen-coated surface only. The data for each assay is the mean of two or more determinations. The dotted line indicates the threshold for a positive test.

  1. Supplementary Material