A Comparison of Anti-Nuclear Antibody Quantification Using Automated Enzyme Immunoassays and Immunofluorescence Assays

Table 2

Comparison of the immunofluorescence patterns and titers of HEp-2000 ANA testing at two independent laboratories.

Secondary laboratory	NEG	POS^?	CEN^?	CEN	HOM^+	HOM	NUC^?	NUC and HOM	NUC	SPE^?	SPE^+	SPE	MIT
NEG	22	3	1		11	𝟑			𝟐		𝟐		1
CEN > 1280				1
CEN > 1280 and MEM				1
CEN > 1280 and SPE > 1280				1
HOM^+						1
HOM > 1280						2
HOM > 1280 and SPE > 1280						1
HOM 1280						1
HOM 160					1
NUC^++									1
NUC 160	𝟏					𝟏			2
NUC 320									1
NUC 320 and SPE 160							1
NUC 640						𝟏		1	1
SPE 320										1
SPE												1
SPE > 1280												8
SPE^+++												1
MIT > 1280		1

Primary laboratory: Gentofte Hospital, and secondary laboratory: Statens Serum Institut; NEG: negative; POS: positive; CEN: centromere; HOM: homogeneous; NUC: nuclear; SPE: speckled; MIT: mitochondria; ?: uncertain reaction; +: weak reaction; ++: intermediate reaction, and +++: strong reaction. Underlining and bold font indicate agreement, italics indicate acceptable agreement, and double underlining indicates disagreement.