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Autoimmune Diseases
Volume 2016, Article ID 9324315, 7 pages
http://dx.doi.org/10.1155/2016/9324315
Research Article

Infiltrating CD16+ Are Associated with a Reduction in Peripheral CD14+CD16++ Monocytes and Severe Forms of Lupus Nephritis

1Grupo de Inmunología Celular e Inmunogenética, GICIG, Universidad de Antioquía, Medellín, Colombia
2Grupo de Reumatología, Universidad de Antioquía, Medellín, Colombia
3Grupo de Patología Renal y Trasplante, Universidad de Antioquía, Medellín, Colombia

Received 27 July 2016; Revised 27 October 2016; Accepted 30 October 2016

Academic Editor: Ricard Cervera

Copyright © 2016 Anabel Barrera García et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Our aim was to characterize glomerular monocytes (Mo) infiltration and to correlate them with peripheral circulating Mo subsets and severity of lupus nephritis (LN). Methods. We evaluated 48 LN biopsy samples from a referral hospital. Recognition of Mo cells was done using microscopic view and immunohistochemistry stain with CD14 and CD16. Based on the number of cells, we classified LN samples as low degree of diffuse infiltration (<5 cells) and high degree of diffuse infiltration (≥5 cells). Immunophenotyping of peripheral Mo subsets was done using flow cytometry. Results. Mean age was years and the mean SLEDAI was . The most common SLE manifestations were proteinuria (91%) and hypocomplementemia (75%). Severe LN was found in 70% of patients (Class III, 27%; Class IV, 43%). Severe LN patients and patients with higher grade of CD16+ infiltration had lower levels of nonclassical (CD14+CD16++) Mo in peripheral blood. Conclusions. Our results might suggest that those patients with more severe forms of LN had a higher grade of CD14+CD16+ infiltration and lower peripheral levels of nonclassical (CD14+CD16++) Mo and might reflect a recruitment process in renal tissues. However, given the small sample, our results must be interpreted carefully.