Table of Contents
Advances in Ecology
Volume 2014, Article ID 731291, 4 pages
http://dx.doi.org/10.1155/2014/731291
Research Article

Evidence against the Presence of Wolbachia in a Population of the Crayfish Species Procambarus clarkii

Department of Biology, New Mexico State University, P.O. Box 30001/MSC 3AF, Las Cruces, NM 88003, USA

Received 29 July 2014; Accepted 3 December 2014; Published 22 December 2014

Academic Editor: Sveinn Are Hanssen

Copyright © 2014 Daniel A. Heneghan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Wolbachia is a genus of intracellular alpha-Proteobacteria that is maternally inherited and is capable of inducing a variety of reproductive alterations in host species. The host range of Wolbachia is not determined completely but is known to contain a number of arthropod taxa, including crustaceans. Wolbachia has not been reported in crayfish, but sampling has been limited to date. We examine a species of crayfish, Procambarus clarkii, for Wolbachia infection using a suite of Wolbachia-specific primers in PCR assays. All specimens yielded negative results for Wolbachia infection and mathematical analysis of sample size shows a near 100% probability of detection for populations with greater than 0.1% infection rate.