Marrow Stromal Cell Infusion Rescues Hematopoiesis in Lethally Irradiated Mice despite Rapid Clearance after Infusion
Figure 1
Impact of MSC infusion on survival of C57/Bl6 mice. (a) Determination of lethal dose of radiation for C57/Bl6 mice when radiation delivered with the RS2000 X-ray irradiator. Adult female mice 6 to 8 weeks in age were radiated with 600, 700, or 800 cGy radiation ( each) in two divided doses survival rate calculated at 7 weeks after radiation. None of the animals that received 700 or 800 cGy radiation survived past 4 weeks from day of radiation. 4 of the 5 mice in the 600 cGy radiation group survived past 7 weeks from day of radiation (). (b) Survival of mice receiving MSCs (clones or primary) when compared to control animals. Survival was studied in adult female mice receiving 700 cGy radiation followed by one of the following interventions: cloned MSCs (cMSC, ), primary MSCs (pMSC, ) or PBS (). Survival was significantly better for the cMSC (53%) and pMSC (60%) groups when compared to PBS ( values of 0.017 and 0.041, resp. with logrank test) at 7 weeks after radiation.
