Advances in Hematology / 2012 / Article / Fig 1

Research Article

EBV Reactivation and Chromosomal Polysomies: Euphorbia tirucalli as a Possible Cofactor in Endemic Burkitt Lymphoma

Figure 1

(a) Cord-blood-derived cells were treated with different concentration of E. tirucalli for five days, and proliferation has been monitored. Methanol-treated cells, with no E. tirucalli extract, have been used as a control. A dose-dependent decrease of proliferation rate is observed in E. tirucalli-treated cells ( ). The graph is representative of three different experiments. Error bars represent standard deviation between duplicates. (b) Cell cycle analysis by FACS on untreated (upper part) and E. tirucalli-treated cells (lower part). Tables indicate the percentage of cells in each cell cycle stages, where M1 indicates total number of dead cells (apoptotic and necrotic cells), M2 indicates G0/G1, M3 cells in S phase, and M4 the G2/M phase. Treated cells show a higher number of the M1 fraction. (c) Electrophoresis on agarose gel of untreated (lanes 1-2) and E. tirucalli-treated cells (lanes 3-4). No DNA laddering indicative of cell death by apoptosis is visible. (d) ICC for caspase 3. No caspase activation is detected following E. tirucalli treatment.

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