EBV Reactivation and Chromosomal Polysomies: Euphorbia tirucalli as a Possible Cofactor in Endemic Burkitt Lymphoma
(a) Cord-blood-derived cells were treated with different concentration of E. tirucalli for five days, and proliferation has been monitored. Methanol-treated cells, with no E. tirucalli extract, have been used as a control. A dose-dependent decrease of proliferation rate is observed in E. tirucalli-treated cells (). The graph is representative of three different experiments. Error bars represent standard deviation between duplicates. (b) Cell cycle analysis by FACS on untreated (upper part) and E. tirucalli-treated cells (lower part). Tables indicate the percentage of cells in each cell cycle stages, where M1 indicates total number of dead cells (apoptotic and necrotic cells), M2 indicates G0/G1, M3 cells in S phase, and M4 the G2/M phase. Treated cells show a higher number of the M1 fraction. (c) Electrophoresis on agarose gel of untreated (lanes 1-2) and E. tirucalli-treated cells (lanes 3-4). No DNA laddering indicative of cell death by apoptosis is visible. (d) ICC for caspase 3. No caspase activation is detected following E. tirucalli treatment.
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