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Advances in Hematology
Volume 2012 (2012), Article ID 460801, 7 pages
Grey Zone Lymphomas: Lymphomas with Intermediate Features
1Institute of Pathology, University Hospital Basel, 4031 Basel, Switzerland
2AP-HP, Groupe Henri Mondor-Albert Chenevier, Département de Pathologie, Hôpital Henri Mondor, 51 Avenue du Maréchal de Lattre de Tassigny, 94010 Créteil, France
3Université Paris-Est, Faculté de Médecine, UMR-S 955, 94010 Créteil, France
4INSERM, Unité U955, 94010 Créteil, France
Received 25 October 2011; Revised 10 January 2012; Accepted 20 January 2012
Academic Editor: Kikkeri N. Naresh
Copyright © 2012 Sylvia Hoeller and Christiane Copie-Bergman. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The current classification of lymphoid neoplasms is based on clinical information, morphology, immunophenotype, and molecular genetic characteristics. Despite technical and scientific progress, some aggressive B-cell lymphomas with features overlapping between two different types of lymphomas remain difficult to classify. The updated 2008 World Health Organization (WHO) classification of Tumours of the Hematopoietic and Lymphoid Tissues has addressed this problem by creation of two new provisional categories of B-cell lymphomas, unclassifiable; one with features intermediate between diffuse large B-cell lymphoma and classical Hodgkin lymphoma and the second with features intermediate between diffuse large B-cell lymphoma and Burkitt lymphoma. We review here the diagnostic criteria of these two provisional entities and discuss new scientific findings in light of the 2008 WHO classification.
The current classification of lymphoid neoplasms is based on clinical information morphology, immunophenotype, and molecular genetic characteristics. Most lymphomas can be accurately classified. However, some lymphomas present with features transitional between diffuse large B-cell lymphomas (DLBCLs) and classical Hodgkin lymphoma (cHL) or DLBCL and Burkitt lymphoma (BL), and these are difficult to classify . These lymphomas have been reported in the literature using different terms, such as borderline lymphomas, B-cell lymphomas unclassifiable, atypical Burkitt lymphoma, Burkitt-like lymphomas, or gray zone lymphomas. The term “Gray Zone Lymphoma” was firstly used in 1998 at the “Workshop on Hodgkin’s disease and related diseases” to designate lymphomas at the border of cHL and other entities . This term was then further extended to lymphomas with overlapping features between BL and DLBCL. The 2008 updated WHO classification of Tumours of the Hematopoietic and Lymphoid Tissues proposed to assign these gray zone lymphomas to provisional categories called B-cell lymphomas unclassifiable with features intermediate between DLBCL and cHL (BCLu-DLBCL/cHL) and B-cell lymphomas unclassifiable with features intermediate between DLBCL and BL (BCLu-DLBCL/BL) . The reason to create these provisional categories is to enable to collect for further studies and to maintain the “purity” of well-defined categories. This would be particularly relevant for conducting clinical studies. This paper focuses on these two provisional entities introduced in the 2008 WHO classification of Tumours of the Hematopoietic and Lymphoid Tissues.
2. B-Cell Lymphoma, Unclassifiable, with Features Intermediate between Diffuse Large B-Cell Lymphoma and Classical Hodgkin Lymphoma (BCLu-DLBCL/cHL)
Primary mediastinal diffuse large B-cell lymphoma (PMBCL) and classical Hodgkin lymphoma of nodular sclerosing subtype (cHL-NS) have clinical, histopathological, and molecular similarities (Table 1). Both lymphomas present as an anterior mediastinal mass with involvement of the thymus and/or supraclavicular lymph nodes and affect preferentially young women. Median age of presentation is slightly older in PMBCL (35 years) than in cHL-NS (30 years) . The histopathological features of PMBCL include a diffuse proliferation of large cells with clear abundant cytoplasm and fine compartmentalizing sclerosis. Reed-Sternberg-like cells may be present [5, 6], and distinction from cHL-NS can sometimes be difficult. The neoplastic cells in PMBCL express B-cell markers (CD20, CD79a, CD19, PAX5) and lack expression of HLA class I antigens and surface immunoglobulin (Ig). However, expression of Ig-associated transcription factors BOB1, OCT2, and PU1 is preserved in contrast to cHL [7, 8]. CD30 is expressed in 70% of cases and tumour cells are typically CD23 positive. Seventy per cent of PMBCL and 10% of cHL express the MAL protein linking them histogenetically to the thymic asteroid medullary B cells [9, 10]. EBV is absent in PMBCL.
On gene expression profiling studies, the PMBCL gene signature differs from that of germinal centre B-cell-like and activated B-cell-like DLBCL. Highly expressed genes include MAL, Interleukine 4 induced gene 1 (IL4I1), TARC, NFkB2, and PDL1/L2 [11, 12]. Interestingly, the PMBCL gene signature appeared to be more related to cHL gene signature as both represent downregulation BCR pathway signalling, constitutive NF-kappa B activation, activation of the cytokine-JAK-STAT pathway, high expression of extracellular matrix elements, overexpression of the TNF family members, and aberrant activation of the P13K/AKT pathway [11–17]. Recent studies have highlighted many genetic similarities as well. Both entities show gains at 2p15 (REL locus) and 9p24 (JAK2 locus) and breaks at CIITA (38% of PMBCL and 15% of cHL) [18–20]. The presence of CIITA rearrangement significantly correlates with shorter disease-specific survival for patients with PMBCL . Altogether, these features point to a similar histogenesis.
In recent years, cases with morphological and immunophenotypic features transitional between PMBCL and the nodular sclerosis subtype of cHL have been reported. These cases, which were initially referred to as “gray zone lymphomas,” were assigned in the 2008 WHO classification to a provisional category designated B-cell lymphoma, unclassifiable, with features intermediate between DLBCL and cHL (BCLu-DLBCL/cHL). These lymphomas with intermediate features have been reported mostly from Western countries, and they seem to be less frequent in sub-Saharan Africa and Asia . BCLu-DLBCL/cHL usually presents with mediastinal manifestations, but also include occasional cases involving nonmediastinal lymph node sites. Involvement of lung (by direct extension), liver, spleen, and bone marrow are documented. In contrast to PMBCL, nonlymphoid organs are rarely infiltrated . Interestingly, these lymphomas are probably more frequent in young men and have a more aggressive clinical course and poorer outcome than either cHL or PMBCL [21, 22]. From the morphological point of view BCLu-DLBCL/cHL shows typically sheet-like, confluent growth of pleomorphic tumour cells embedded in a diffusely fibrotic stroma. The majority of tumour cells classically resemble lacunar cells and Hodgkin cells. However, the tumour shows marked variation of morphological aspects ranging from cHL to DLBCL/PMBCL in the same tumour. There is usually a sparse inflammatory infiltrate present with only scattered eosinophils, lymphocytes, and histiocytes. Typically necrotic areas do not include neutrophilic infiltrates. Immunohistochemically, B-cell program is usually preserved in the tumour cells with expression of the transcription factors PAX5, OCT-2, and BOB.1, but this profile is accompanied by expression of typical “cHL markers” like CD15 and CD30. Surface Ig expression is absent. MAL, a typical marker for PMBCL , is expressed in at least a proportion of cases . Diagnostic criteria include, for example, cases morphologically resembling PMBCL but with strong expression of CD15, absence of CD20 or presence of EBV . Cases rich in tumour cells resembling cHL, which are strongly positive for CD20 and/or other B-cell markers, are also included in this category .
The existence of composite (cHL and PMBCL at the time of diagnosis) or sequential/metachronous lymphomas (cHL following a diagnosis of PMBCL or vice versa) suggests that some lymphomas in the mediastinum show lineage plasticity with a shift over time toward the one or the other entity  which may be due to epigenetic and not genetic mechanisms. Based on this consumption Eberle et al. studied the DNA methylation status which is the best established epigenetic marker so far from 10 mediastinal gray zone lymphomas (MGZLs) compared to 10 cHL-NS, 10 PMBCL, and 10 nodal DLBCL cases . MGZL cases had epigenetic profiles intermediate to cHL and PMBCL but clearly distinct from DLBCL. PMBCL and cHL-NS presented with distinct methylation signatures. cHL-NS showed presence of de novo hypermethylation and absence of de novo hypomethylation within CpG islands and in a fraction of promoters outside CpG islands. These results are in line with other studies suggesting that the development of Reed-Sternberg cells may be due to gene silencing by DNA methylation [25, 26]. In contrast, PMBCL showed both de novo hypermethylation and hypomethylation. Interestingly, HOXA5 hypomethylation was exclusively found in MGZL, and the biological relevance of this finding remains to be explored.
Eberle and colleagues studied the genetic features of 27 MGZL and 6 mediastinal composite or synchronous/metachronous lymphomas by fluorescence in situ hybridization. They demonstrated amplification in 2p16.1 (REL/BCL11A locus) and alterations in 9p24.1 (JAK2/PD2 locus) in 33% and 55% of the patients, respectively. In addition, rearrangement of the CIITA locus at 16p13.13 and gains of 8p24 (MYC) were both observed in 27% of the cases each . These very recent findings underline the plasticity of mediastinal BCLu-DLBCL/cHL not only on morphologic and immunophenotypic but also on molecular grounds.
Identification of BCLu-DLBCL/cHL will hopefully enable to better characterize them and above all to develop an optimal therapeutic approach. The clinical management of these patients is actually a challenge for clinicians as cHL and PMBCL require different therapies. Due to the rarity of the disease and the complexity of diagnostic criteria, the optimal therapy for these patients remains unclear. In a small series of patients with mediastinal BCLu-DLBCL/cHL, Traverse-Glehen et al. suggested that patients might benefit better from therapy designed for aggressive B-cell non-Hodgkin lymphoma than those applied to patients with cHL . However, these results need validation on a larger series of patients.
3. B-Cell Lymphoma, Unclassifiable, with Features Intermediate between Diffuse Large B-Cell Lymphoma and Burkitt Lymphoma (BCLu-DLBCL/BL)
In order to understand the concept of this new category of lymphoma, we will briefly review the diagnostic criteria for Burkitt lymphoma (BL) according to the updated WHO classification of lymphoid neoplasms.
BL includes three epidemiologic variants, the endemic (so-called African type with 100% EBV association), the sporadic, and the immunodeficiency-associated (mostly HIV infected patients). Additionally, cases presenting with a leukemic picture (formerly known as L3 ALL) and without significant lymphadenopathy are also included. The atypical/Burkitt-like and the plasmacytoid variants are no longer mentioned .
The diagnostic criteria for BL are quite strict. Morphologically, the tumour cells are medium sized, with monotonous cytology presenting with round nuclei with finely clumped and dispersed chromatin and multiple basophilic paracentrally situated nucleoli. The cytoplasm is deeply basophilic and usually contains lipid vacuoles (which may better be seen in imprints). The growth pattern is diffuse, and the tumour cells often seem to grow in a cohesive way. The proliferation fraction is extremely high (>90%) with many mitotic figures accompanied by a high fraction of apoptosis and often a starry sky pattern due to the background occurrence of tingible body macrophages . In contrast with previous classification, increased nuclear irregularity, slight nuclear pleomorphism, and/or more prominent, single nucleoli are allowed if the immunophenotype and the molecular characteristics fit with the diagnosis of BL. These lymphomas previously classified as “Burkitt-like” or “atypical Burkitt” lymphoma are now included in the “Burkitt lymphoma” category, and hence the terms “Burkitt-like” or “atypical Burkitt” lymphoma should not be used any longer. This approach is supported by molecular studies, which have revealed that the cases classified morphologically as “atypical” BL have a molecular signature similar to classical BL [23, 28].
The immunohistophenotype required for the diagnosis of BL is strong CD10 and BCL6 positivity, negativity for BCL2, and a Ki67 index of near 100% (at least 90%) . Weak positivity for BCL2 is accepted, but strong expression for BCL2 and a proliferation fraction below 90% are strong contraindications for a diagnosis of BL .
On genetics, most cases show rearrangement of MYC at 8q24 to the IG heavy chain (14q32) or less frequent to the kappa (22q11) or lambda (2p12) light chain loci. The breakpoints are different in endemic and sporadic BL. Endemic BLs present with breakpoints occurring within the VJ region of the IGH locus, while sporadic BL mainly present with breakpoints occurring within IGH switch regions of the IGH locus, which may point to the differing maturation status of the two types [29, 30]. Importantly, up to 10% of BL may lack a MYC gene translocation by FISH. To date it is not clear if this is due to a failure of detection or if such lymphomas really exist. However, MYC-negative BL in adults is characterised by downregulation of microRNA hsa-mir-34b and recurrent duplications of chromosome 11 [28, 31, 32]. However, these MYC-negative BLs have to be completely typical on morphological and immunophenotypic grounds to be classified as BL although this opinion is not shared by all authors .
However, in view of the strict diagnostic criteria, there remain cases, which are not completely typical, either on morphological and/or immunohistochemical/genetic grounds and therefore are difficult to assign to either BL or DLBCL category (classical common and distinguishing features between these two entities are listed in Table 2). Gene expression profiling (GEP) studies reveal that though the GEP signature of BL and DLBCL are distinct, in a proportion of B-cell lymphomas the GEP signature is intermediate between BL and DLBCL . Such cases were diagnosed either as BL or DLBCL on morphologic and immunohistochemical grounds by expert hematopathologists, and possibly inappropriate therapy was rendered for some patients. Based on these observations, the recent WHO classification created a provisional entity, B-cell lymphoma, unclassifiable, with features intermediate between DLBCL and BL (BCLu-DLBCL/BL). The reasons for the creation of this new entity are mostly similar to those of BCLu-DLBCL/cHL. Firstly, it intends to collect cases with intermediate features under the same name, and secondly it segregates “clean” BL and DLBCL, which is extremely helpful for clinical trials. On the other hand, it also creates difficulties to clinicians, as the therapeutic strategies differ greatly in adults between BL and DLBCL, and there has been no consensus, on how to treat patients with BCLu-DLBCL/BL.
BCLu-DLBCL/BL are relatively rare and mainly diagnosed in adults . They represent up to 5% of adult aggressive B-cell lymphomas and usually occur in extranodal sites sometimes associated with leukemic involvement . By definition, BCLu-DLBCL/BL harbour intermediate morphological and immunohistochemical features between BL and DLBCL . They may be medium or large cells, usually with a high proliferation fraction and starry sky pattern, with an atypical immunophenotype (lack of CD10 and/or strong BCL2 expression) that precludes the diagnosis of BL. Most of them are of germinal centre subtype with expression of CD10, BCL6 and lack of MUM1 .
Cytogenetic characterization of these BCLu has shown that a proportion of them harbours a complex karyotype with two main genetic events—usually cMYC alterations together with BCL2 and/or BCL6, less commonly CCND1 rearrangements, designated the so-called “double hit” lymphomas (DHL). Some patients may have a previous history of low-grade lymphoma such as follicular lymphoma, CLL, or mantle cell lymphoma, and the acquisition of cMYC alteration may represent a secondary genetic event .
Importantly, lymphomas with a typical DLBCL morphology that have a MYC breakpoint are excluded from the category of BCLu-DLBCL/BL. Up to 15% of DLBCL have MYC translocations [23, 34], and they are generally associated with an inferior outcome [35, 36].
The clinical evolution of patients with double-hit lymphomas is dramatic with a median survival of 4.5 months, and they are usually resistant to either conventional CHOP-like regimens or to intensive therapy used to treat BL. However, factors associated with a better survival have been identified, which include non-IGH MYC partner, BCL2 protein expression, and rituximab inclusive chemotherapy .
Altogether, this category of lymphoma appears heterogeneous and remains difficult to diagnose in day-to-day practice based on morphological and immunohistochemical grounds. Interphase FISH with BCL2, BCL6, and cMYC DNA probes provides a useful diagnostic tool to identify these DHL. Adult cases in which BL or DLBCL/BL is a diagnostic consideration should be tested for MYC, BCL2, and BCL6 rearrangements, and if MYC break is associated with BCL2 and/or BCL6 rearrangements, the case should be classified as DLBCL/BL irrespective of other features .
A very recent paper from the group of Reiner Siebert  reviewed the “grey zone” between BL and DLBCL from a genetic perspective. This paper aims to clarify the different definitions of intermediate lymphomas and to propose a subclassification based on genetic aberrations. The “intermediate lymphoma” group from GEP studies and the BCLu-DLBCL/BL from the WHO classification are by far not identical. The intermediate group of GEP is defined of a group of lymphomas not meeting the profiling of either molecular BL or for molecular DLBCL. Therefore, this category represents a wastebasket for all lymphomas, which do not fit into the two molecularly defined entities. On the other hand, the BCLu-DLBCL/BL entity defined by the WHO contains all lymphomas, which does not meet the criteria of either BL or DLBCL on morphologic, immunohistochemical, and classical genetic grounds and represents a heterogeneous group of diseases. However, the following aggressive B-cell lymphomas are excluded from BCLu-DLBCL/BL: cases with typical DLBCL morphology with a very high proliferation index, typical DLBCL with a MYC translocation, and typical BL in which a MYC rearrangement cannot be demonstrated and those with IG-MYC rearrangement as the only abnormality, since they probably correspond to real BL with atypical morphology. Salaverria and Siebert  proposed a simple approach based predominantly on age and genetic aberrations to classify these aggressive B-cell lymphomas into biologically meaningful and clinically recognizable subgroups.
In children, the classification into BCLu-DLBCL/BL has currently no influence on therapy or outcome and either intermediate lymphomas according to both GEP and WHO classification are infrequent in patients under the age of 18 years [3, 39] and do not seem to have an adverse prognosis. Almost all childhood “intermediate lymphoma” present with IG-MYC fusion, and BCL2 breaks are almost always absent. Therefore, it seems that in children “intermediate lymphomas” represent rather true biologic BL, which were classified as “intermediate” in the GEP due to secondary aberrations . In contrast some morphological DLBCLs in children show a GEP signature of molecular BL with more than half of them being IG-MYC positive, suggesting that the presence of the MYC fusion is mostly responsible for its given molecular signature. Since the MYC fusion is very likely to be the first event in lymphoma development, complex karyotypes are indicators of disease progression and inferior outcome and do not indicate an IG-MYC fusion as a secondary event in children.
In adult patients the situation is rather different and the subclassification of aggressive B-cell lymphomas has a true impact on treatment decisions and prognosis. Salaverria and Siebert  suggest that adult aggressive B-cell lymphomas lacking typical BL morphology and phenotype can be classified thereafter into four different genetic subcategories according to their MYC status as follows.
(1) IG-MYC-Positive Mature Aggressive Lymphomas with Simple Karyotype Lacking Typical BL Morphology and/or Phenotype. These cases represent a spectrum ranging from cases that would be classified as BL to up to DLBCL based on the WHO classification, since the WHO considers morphology, immunophenotype, and genetic characteristics as being equally relevant . Since the molecular BL signature can also be found in classical DLBCL cases , this points to the fact that such cases might be candidates for this molecular BL group.
(2) IG-MYC-Positive Mature Aggressive B-Cell Lymphomas with Complex Karyotype, Lacking Typical BL Morphology, and/or Phenotype Carrying a High Genetic Complexy. These cases can correspond to BL with progression or DLBCL with secondary MYC break. However, like in the first group it is not easy to set a cutoff between complex and simple karyotype, since there a standard reference method is not defined.
(3) Non-IG-MYC-Positive Mature Aggressive B-Cell Lymphomas. MYC translocations can involve partners other than the IG heavy or light chain loci. Those translocations are almost exclusively considered as secondary events. These translocations are exceedingly rare in BL but represent up to half of MYC translocations in BCLu-DLBCL/BL [3, 28]. Those cases are probably cases with a different primary genetic event (BCL2, BCL6 break, or others) and acquire the MYC-break secondarily and may develop very complex karyotypes.
(4) Double Hit-Positive Mature Aggressive B-Cell Lymphomas. These lymphomas carry either IG-MYC fusion or non-IG-MYC fusion in combination with either BCL2 and/or BCL6 breaks. By definition these lymphomas would also conform to the previously defined group. But this specific group comprises of >30% of MYC-translocation-positive lymphomas in elderly patients and has an aggressive clinical course. However, Salaverria and Siebert  pointed out that further studies are needed to clarify: if double hit lymphomas are different from other MYC-positive non-BL cases and if the different types of combinations (DLBCL/BL or DLBCL with BCL2 and/or BCL6 break and IG-MYC or non-IG-MYC fusion) influence the outcome and behavior of these lymphomas.
In addition, there are also MYC-translocation-negative aggressive B-cell lymphomas with features intermediate between BL and DLBCL. Here the “intermediate” status is defined according to the WHO criteria by histology and immunophenotype. This group probably represents a heterogeneous group of mainly DLBCL, but little is known about this category to date. Salaverria and Siebert  suggest that this genetic classification should be tested for reproducibility and clinical impact in future clinical trials.
In conclusion, since the publication of the 2008 WHO classification, several groups have tried to better characterize these two new provisional entities of B-cell lymphomas, unclassifiable with intermediate features. Eberle and colleagues [24, 27] have shed new insights on the epigenetic and cytogenetic characteristics of BCLu-DLBCL/cHL which highlight the plasticity of the tumour cells and the molecular continuum between PMBCL and cHL. Regarding the second category of B-cell lymphoma, BCLu-DLBCL/BL, the situation is more complex. This category appears extremely heterogeneous and remains difficult to diagnose in day-to-day practice based on morphological and immunohistochemical features. The genetic approach proposed by Salaverria is interesting and supports the fact that the detection of chromosomal abnormalities in the diagnostic workup of aggressive B-cell lymphomas is becoming increasingly important.
Further studies are needed to better define the diagnostic criteria of BCLu-DLBCL/BL and allow clinicians to conduct clinical trials to define the optimal therapy which remains unclear to date.
- L. Quintanilla-Martinez, D. de Jong, A. de Mascarel et al., “Gray zones around diffuse large B cell lymphoma. Conclusions based on the workshop of the XIV meeting of the European Association for Hematopathology and the Society of Hematopathology in Bordeaux, France,” Journal of Hematopathology, vol. 2, no. 4, pp. 211–236, 2009.
- T. Rudiger, E. S. Jaffe, G. Delsol et al., “Workshop report on Hodgkin's disease and related diseases ('grey zone' lymphoma),” Annals of Oncology, vol. 9, no. 5, supplement, pp. S31–S38, 1998.
- S. H. C. E. Swerdlow, N. L. Harris, et al., WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues, IARC, Lyon, France, 2008.
- C. Steidl and R. D. Gascoyne, “The molecular pathogenesis of primary mediastinal large B-cell lymphoma,” Blood, vol. 118, no. 10, pp. 2659–2669, 2011.
- L. Lamarre, J. O. Jacobson, A. C. Aisenberg, and N. L. Harris, “Primary large cell lymphoma of the mediastinum. A histologic and immunophenotypic study of 29 cases,” American Journal of Surgical Pathology, vol. 13, no. 9, pp. 730–739, 1989.
- S. Suster and C. A. Moran, “Primary thymic epithelial neoplasms showing combined features of thymoma and thymic carcinoma: a clinicopathologic study of 22 cases,” American Journal of Surgical Pathology, vol. 20, no. 12, pp. 1469–1480, 1996.
- T. F. E. Barth, F. Leithäuser, S. Joos, M. Bentz, and P. Möller, “Mediastinal (thymic) large B-cell lymphoma: where do we stand?” Lancet Oncology, vol. 3, no. 4, pp. 229–234, 2002.
- S. Hoeller, D. Zihler, I. Zlobec et al., “BOB.1, CD79a and cyclin e are the most appropriate markers to discriminate classical Hodgkin's lymphoma from primary mediastinal large B-cell lymphoma,” Histopathology, vol. 56, no. 2, pp. 217–228, 2010.
- L. Quintanilla-Martinez and F. Fend, “Mediastinal gray zone lymphoma,” Haematologica, vol. 96, no. 4, pp. 496–499, 2011.
- C. Copie-Bergman, A. Plonquet, M. A. Alonso et al., “Mal expression in lymphoid cells: further evidence for mal as a distinct molecular marker of primary mediastinal large B-cell lymphomas,” Modern Pathology, vol. 15, no. 11, pp. 1172–1180, 2002.
- K. J. Savage, S. Monti, J. L. Kutok et al., “The molecular signature of mediastinal large B-cell lymphoma differs from that of other diffuse large B-cell lymphomas and shares features with classical Hodgkin lymphoma,” Blood, vol. 102, no. 12, pp. 3871–3879, 2003.
- A. Rosenwald, G. Wright, K. Leroy et al., “Molecular diagnosis of primary mediastinal B cell lymphoma identifies a clinically favorable subgroup of diffuse large B cell lymphoma related to Hodgkin lymphoma,” Journal of Experimental Medicine, vol. 198, no. 6, pp. 851–862, 2003.
- F. Feuerhake, J. L. Kutok, S. Monti et al., “NFκB activity, function, and target-gene signatures in primary mediastinal large B-cell lymphoma and diffuse large B-cell lymphoma subtypes,” Blood, vol. 106, no. 4, pp. 1392–1399, 2005.
- C. Guiter, I. Dusanter-Fourt, C. Copie-Bergman et al., “Constitutive STAT6 activation in primary mediastinal large B-cell lymphoma,” Blood, vol. 104, no. 2, pp. 543–549, 2004.
- C. Renné, K. Willenbrock, J. I. Martin-Subero et al., “High expression of several tyrosine kinases and activation of the PI3K/AKT pathway in mediastinal large B cell lymphoma reveals further similarities to Hodgkin lymphoma,” Leukemia, vol. 21, no. 4, pp. 780–787, 2007.
- O. Ritz, C. Guiter, F. Castellano et al., “Recurrent mutations of the STAT6 DNA binding domain in primary mediastinal B-cell lymphoma,” Blood, vol. 114, no. 6, pp. 1236–1242, 2009.
- O. Ritz, C. Guiter, K. Dorsch et al., “STAT6 activity is regulated by SOCS-1 and modulates BCL-XL expression in primary mediastinal B-cell lymphoma,” Leukemia, vol. 22, no. 11, pp. 2106–2110, 2008.
- S. Joos, M. I. Otaño-Joos, S. Ziegler et al., “Primary mediastinal (thymic) B-cell lymphoma is characterized by gains of chromosomal material including 9p and amplification of the REL gene,” Blood, vol. 87, no. 4, pp. 1571–1578, 1996.
- S. Wessendorf, T. F. E. Barth, A. Viardot et al., “Further delineation of chromosomal consensus regions in primary mediastinal B-cell lymphomas: an analysis of 37 tumor samples using high-resolution genomic profiling (array-CGH),” Leukemia, vol. 21, no. 12, pp. 2463–2469, 2007.
- C. Steidl, S. P. Shah, B. W. Woolcock et al., “MHC class II transactivator CIITA is a recurrent gene fusion partner in lymphoid cancers,” Nature, vol. 471, no. 7338, pp. 377–383, 2011.
- A. Traverse-Glehen, S. Pittaluga, P. Gaulard et al., “Mediastinal gray zone lymphoma: the missing link between classic Hodgkin's lymphoma and mediastinal large B-cell lymphoma,” American Journal of Surgical Pathology, vol. 29, no. 11, pp. 1411–1421, 2005.
- J. F. García, M. Mollejo, M. Fraga et al., “Large B-cell lymphoma with Hodgkin's features,” Histopathology, vol. 47, no. 1, pp. 101–110, 2005.
- R. P. Hasserjian, G. Ott, K. S. Elenitoba-Johnson, et al., “Commentary on the WHO classification of tumors of lymphoid tissues (2008): “Gray zone” lymphomas overlapping with Burkitt lymphoma or classical Hodgkin lymphoma,” Journal of Hematopathology, vol. 2, no. 2, pp. 89–95, 2009.
- F. C. Eberle, J. Rodriguez-Canales, L. Wei et al., “Methylation profiling of mediastinal gray zone lymphoma reveals a distinctive signature with elements shared by classical Hodgkin's lymphoma and primary mediastinal large B-cell lymphoma,” Haematologica, vol. 96, no. 4, pp. 558–566, 2011.
- A. Ehlers, E. Oker, S. Bentink, D. Lenze, H. Stein, and M. Hummel, “Histone acetylation and DNA demethylation of B cells result in a Hodgkin-like phenotype,” Leukemia, vol. 22, no. 4, pp. 835–841, 2008.
- A. Ushmorov, O. Ritz, M. Hummel et al., “Epigenetic silencing of the immunoglobulin heavy-chain gene in classical Hodgkin lymphoma-derived cell lines contributes to the loss of immunoglobulin expression,” Blood, vol. 104, no. 10, pp. 3326–3334, 2004.
- F. C. Eberle, I. Salaverria, C. Steidl et al., “Gray zone lymphoma: chromosomal aberrations with immunophenotypic and clinical correlations,” Modern Pathology, vol. 24, no. 12, pp. 1586–1597, 2011.
- M. Hummel, S. Bentink, H. Berger et al., “A biologic definition of Burkitt's lymphoma from transcriptional and genomic profiling,” The New England Journal of Medicine, vol. 354, no. 23, pp. 2419–2430, 2006.
- J. E. J. Guikema, C. de Boer, E. Haralambieva et al., “IGH switch breakpoints in Burkitt lymphoma: exclusive involvement of noncanonical class switch recombination,” Genes Chromosomes and Cancer, vol. 45, no. 9, pp. 808–819, 2006.
- B. Shiramizu, F. Barriga, J. Neequaye et al., “Patterns of chromosomal breakpoint locations in Burkitt's lymphoma: relevance to geography and Epstein-Barr virus association,” Blood, vol. 77, no. 7, pp. 1516–1526, 1991.
- E. G. Boerma, R. Siebert, P. M. Kluin, and M. Baudis, “Translocations involving 8q24 in Burkitt lymphoma and other malignant lymphomas: a historical review of cytogenetics in the light of todays knowledge,” Leukemia, vol. 23, no. 2, pp. 225–234, 2009.
- E. Leucci, M. Cocco, A. Onnis et al., “MYC translocation-negative classical Burkitt lymphoma cases: an alternative pathogenetic mechanism involving miRNA deregulation,” Journal of Pathology, vol. 216, no. 4, pp. 440–450, 2008.
- S. M. Aukema, R. Siebert, E. Schuuring et al., “Double-hit B-cell lymphomas,” Blood, vol. 117, no. 8, pp. 2319–2331, 2011.
- J. C. Cigudosa, N. Z. Parsa, D. C. Louie et al., “Cytogenetic analysis of 363 consecutively ascertained diffuse large B- cell lymphomas,” Genes Chromosomes and Cancer, vol. 25, no. 2, pp. 123–133, 1999.
- K. J. Savage, N. A. Johnson, S. Ben-Neriah et al., “MYC gene rearrangements are associated with a poor prognosis in diffuse large B-cell lymphoma patients treated with R-CHOP chemotherapy,” Blood, vol. 114, no. 17, pp. 3533–3537, 2009.
- S. Barrans, S. Crouch, A. Smith et al., “Rearrangement of MYC is associated with poor prognosis in patients with diffuse large B-cell lymphoma treated in the era of rituximab,” Journal of Clinical Oncology, vol. 28, no. 20, pp. 3360–3365, 2010.
- N. A. Johnson, K. J. Savage, O. Ludkovski et al., “Lymphomas with concurrent BCL2 and MYC translocations: the critical factors associated with survival,” Blood, vol. 114, no. 11, pp. 2273–2279, 2009.
- I. Salaverria and R. Siebert, “The gray zone between Burkitt's lymphoma and diffuse large B-cell lymphoma from a genetics perspective,” Journal of Clinical Oncology, vol. 29, no. 14, pp. 1835–1843, 2011.
- W. Klapper, M. Szczepanowski, B. Burkhardt et al., “Molecular profiling of pediatric mature B-cell lymphoma treated in population-based prospective clinical trials,” Blood, vol. 112, no. 4, pp. 1374–1381, 2008.