Genomic Amplification of an Endogenous Retrovirus in Zebrafish T-Cell Malignancies
ZFERV Genomic Organization. The ZFERV retrovirus is comprised of two 695 bp long terminal repeats (LTRs), a 517 bp repeat region (RR) containing 9 direct repeats, and ORFs for 3 proteins: gag, pol, and env . The gag and pol genes share the same reading frame and are predicted to be translated from one transcript by read-through of a stop codon; env uses a different reading frame and is probably a distinct transcript . aCGH probe sites are shown beneath the ZFERV genome schematic: probes found on Zv6 chromosomes 7 (*) and 14 (#) are dispersed throughout ZFERV. One aCGH probe had sequences corresponding to the RR (##), and has multiple binding sites in this area. The 5 remaining aCGH probes map to ORFs. Amplicons from qPCR and qRT-PCR assays are also depicted (not shown to scale). The pol and env qPCR products are 168 and 169 bp, respectively; qRT-PCR products are 225 bp for pol and 234 bp for env.
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