Advances in Hematology / 2012 / Article / Fig 4

Research Article

In Vitro and In Vivo Antitumor Effect of Anti-CD33 Chimeric Receptor-Expressing EBV-CTL against Acute Myeloid Leukemia

Figure 4

Cytotoxicity of anti-CD33.CAR-transduced EBV-CTLs against normal myeloid progenitors. (a) Unmanipulated or anti-CD33.CAR-transduced EBV-CTLs were incubated with bone-marrow-derived CD34+ progenitors at E : T ratio of 1 : 1 for 4 hours. Cells were then seeded in methylcellulose-based medium and after 10 days CFU-GM, CFU-GEMM, and BFU-E were counted. Data shown are mean ± SD of 6 independent experiments. (b) Colonies were collected and characterized by flow cytometry to assess the expression of surface markers specific for each colony type. Data shown are mean ± SD of 6 independent experiments.

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