Figure 4: Effect of transfusion of S1P-loaded fresh and aged RBC on lung endothelial permeability.Twentyfour hours after major blood loss (0.02 mL/gm), mice were treated with LPS. Four hours after LPS treatment, fresh or aged RBCs with or without a preload with SIP (RBC + S1P) were transfused. (a) Evans blue dye (EBD) in the intact lung quantified and (b) representative images of the dye in the lungs demonstrated. Results were analyzed by one-way ANOVA (Bonferroni correction); * versus vehicle.