Management of Adenovirus in Children after Allogeneic Hematopoietic Stem Cell Transplantation
Figure 3
Protocols for generating virus-specific T cells. (a) Donor identified as ADV responder by IFN-γ secretion assay (Miltenyi Biotec, Bergisch Gladbach, Germany). Peripheral blood mononuclear cells (PBMC) isolated and incubated overnight with ADV Hexon protein. Responding cells captured with IFN-γ reagent, anti-IFN-γ microbead and magnetically enriched with CliniMACS (Miltenyi Biotec) [60]. (b) Donor PBMC incubated over 10 days with ADV5 hexon peptide and cytokines. Expanded cells isolated and infused into patients after QA/QC testing [61]. (c) EBV-transformed B cell lines (EBV-LCLs) generated from donor PBMCs by infecting with EBV virus. Donor PBMCs are transfected with Ad5f35pp65 vector (replication-competent adenovirus-negative) and later restimulated several times by EBV-LCLs that have been transduced with the same vector [62].
