RhD Specific Antibodies Are Not Detectable in HLA-DRB1 Mice Challenged with Human RhD Positive Erythrocytes

Figure 4

Immunization of DRB1 mice with KLH-conjugated synthetic RhD peptides induces peptide specific IgG. Mice were immunized with each peptide (P) conjugated to KLH in Freund’s adjuvant as indicated in Materials and Methods (2 mice per peptide). Detection of IgG specific for KLH (a), peptide 1 (b), peptide 2 (c), or peptide 3 (d) was assessed by ELISA. The sera tested were collected at days 28 and 35 (i.e., 14 days after 2nd and 7 days after the 3rd immunization, resp.).

(a)

(b)

(c)

(d)