Research Article

Persistent Polyclonal B Cell Lymphocytosis B Cells Can Be Activated through CD40-CD154 Interaction

Figure 2

PPBL B cells proliferate following low CD154 interaction. Purified B cells isolated from samples obtained from healthy individuals (C-01, C-02, and C-03) and from patients with PPBL (8010, 8011, 8013, 8030, 8031, and 8032) were activated in vitro using a low level (open symbols; dashed line) or a high level (filled symbols; plain line) of CD154 interaction in the presence of cytokines, as indicated in methods. Expansion factors were evaluated using viable cell counts performed in triplicate at the indicated times. Error bars can be smaller than symbols.