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Advances in Hematology
Volume 2016, Article ID 4091461, 6 pages
http://dx.doi.org/10.1155/2016/4091461
Research Article

Influence of Oxidative Stress on Stored Platelets

Department of Biotechnology, Center for Post Graduate Studies, Jain University, No. 18/3, 9th Main, 3rd Block, Jayanagar, Bangalore 560011, India

Received 11 September 2015; Revised 16 December 2015; Accepted 5 January 2016

Academic Editor: Debra A. Hoppensteadt

Copyright © 2016 K. Manasa and R. Vani. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Platelet storage and its availability for transfusion are limited to 5-6 days. Oxidative stress (OS) is one of the causes for reduced efficacy and shelf-life of platelets. The studies on platelet storage have focused on improving the storage conditions by altering platelet storage solutions, temperature, and materials. Nevertheless, the role of OS on platelet survival during storage is still unclear. Hence, this study was conducted to investigate the influence of storage on platelets. Platelets were stored for 12 days at 22°C. OS markers such as aggregation, superoxides, reactive oxygen species, glucose, pH, lipid peroxidation, protein oxidation, and antioxidant enzymes were assessed. OS increased during storage as indicated by increments in aggregation, superoxides, pH, conjugate dienes, and superoxide dismutase and decrements in glucose and catalase. Thus, platelets could endure OS till 6 days during storage, due to the antioxidant defense system. An evident increase in OS was observed from day 8 of storage, which can diminish the platelet efficacy. The present study provides an insight into the gradual changes occurring during platelet storage. This lays the foundation towards new possibilities of employing various antioxidants as additives in storage solutions.