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Advances in Materials Science and Engineering
Volume 2016 (2016), Article ID 6367234, 12 pages
Research Article

Reexamination In Vitro and In Situ of an Antibacterially Modified Experimental Dental Resin Composite with Molecular Methods: A Pilot Study

1Institute of Medical Microbiology and Hospital Hygiene, Medical Faculty, Heinrich-Heine-University, Universitätsstrasse 1, 40225 Düsseldorf, Germany
2Laboratory Bacteriology, Department of Pharmacy, University of “G. D’Annunzio”, Via dei Vestini 31, 66100 Chieti, Italy
3Biological and Medical Research Centre (BMFZ), Heinrich-Heine-University, Universitätsstrasse 1, 40225 Düsseldorf, Germany
4Centre of Dentistry, Section of Periodontics, Heinrich-Heine-University, Moorenstrasse 5, 40225 Düsseldorf, Germany
5Centre of Dentistry, Heinrich-Heine-University, Moorenstrasse 5, 40225 Düsseldorf, Germany
6Center for Dentistry and Oral Medicine (Carolinum), Department of Operative Dentistry, Johann Wolfgang Goethe University, Theodor-Stern-Kai 7, 60596 Frankfurt am Main, Germany

Received 18 January 2016; Revised 25 May 2016; Accepted 2 June 2016

Academic Editor: Jun Liu

Copyright © 2016 Birgit Henrich et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Purpose. To introduce additional methods to detect and to quantify single pathogens in the complex biofilm formation on an antibacterial dental material. Materials and Methods. A conventional (ST) and an antibacterial dental composite (B) were manufactured. In vitro: specimens were incubated with a mixture of early colonizers. Bacterial adhesion was analyzed by TaqMan PCR after 8/24 h. In situ: TaqMan PCR and 16S rRNA Next Generation Sequencing (NGS) were performed. Results. In vitro: after 8 h incubation, B was covered by 58.6% of the bacterial amount that was attached to ST. After 24 h, the amount of attached bacteria to ST remained constant on ST only slightly lower on B. In situ: after 8 h the amount of adhering A. viscosus and S. mitis was prominent on ST and reduced on B. NGS revealed that S. sanguinis, S. parasanguinis, and Gemella sanguinis were the mainly attached species with S. sanguinis dominant on ST and S. parasanguinis and G. sanguinis dominant on B. Conclusions. Initial biofilm formation was altered by B. A shift between actinomycetes and streptococci was observed in situ. TaqMan PCR and 16S rRNA NGS revealed comparable results in situ and demonstrated the usefulness of NGS to characterize complex bacterial communities.