Abstract

Carbonate mineralization microbe is a microorganism capable of decomposing the substrate in the metabolic process to produce the carbonate, which then forms calcium carbonate with calcium ions. By taking advantage of this process, contaminative uranium tailings can transform to solid cement, where calcium carbonate plays the role of a binder. In this paper, we have studied the morphology of mineralized crystals by controlling the mineralization time and adding different concentrations of montmorillonite (MMT). At the same time, we also studied the effect of carbonate mineralized cementation uranium tailings by controlling the amount of MMT. The results showed that MMT can regulate the crystal morphology of calcium carbonate. What is more, MMT can balance the acidity and ions in the uranium tailings; it also can reduce the toxicity of uranium ions on microorganisms. In addition, MMT filling in the gap between the uranium tailings made the cement body more stable. When the amount of MMT is 6%, the maximum strength of the cement body reached 2.18 MPa, which increased by 47.66% compared with that the sample without MMT. Therefore, it is reasonable and feasible to use the MMT to regulate the biocalcium carbonate cemented uranium tailings.

1. Introduction

Biomineralization refers to the process by which organisms produce inorganic minerals through the regulation of biological macromolecules [1]. Microorganisms play an important role in the process of biomineralization, which is the largest and most widely distributed form of life on earth. It has the ability to induce mineral deposition such as carbonic acid/phosphoric acid/sulphate. Microbial-induced mineralization mainly can be defined as the mineralization process caused by the interaction of microbial life activities with the surrounding environment [2]. Soil mineral-microbial interactions are the most basic biogeochemical effects [3, 4]. Microbial metabolic activities play an important role in the elemental cycling, formation, and transformation, as well as the weathering of the earth’s environment [5]. In recent years, the interaction between soil minerals and microbes has become a hot topic in related disciplines. In 2016, Shi et al. published a review in the Journal of Nature Review on microbes [6], which systematically summarized the mechanism of interaction between minerals and microbes, focusing on two basic sciences of energy and extracellular electron transfer. In addition, domestic and foreign scholars have studied the different systems and related mechanism of soil mineral-microbial interaction, involving the microbial adsorption process on the surface of minerals [7, 8]; they also studied the relationship between mineral-microbial-soil contaminants.

At present, there are many studies on the interaction between soil clay minerals and microorganisms, involving clay minerals including montmorillonite (MMT), bentonite, kaolin, hydrotalcite, and illite [9]. Among these clay minerals, MMT and microbial interaction research is one of the hot spots of concern. MMT belongs to the layered silicate clay minerals. MMT has a good adsorption, ion exchange, which is retained on the radionuclide, blocking the migration of nuclides. MMT is widely used for radionuclide pollution controlling and repairing as a green environment-friendly material [10, 11]. At the cellular level, MMT undergoes a series of interactions with microorganisms, including adsorption, aggregation, nucleation, and mineralization [12]. The reason is that the surface of bacteria is easy to dissolve ions or induce a variety of mineral ion nucleation, so as to achieve the purpose of adsorption or mineralization [13]. At the same time, MMT can effectively protect the growth of microorganisms by buffering the pH of the system. In addition, MMT was used as microbial nucleation sites to promote mineral deposition.

In today’s society, with the continuous development of nuclear energy, uranium tailings, and other wastes more and more from the nuclear fuel production process, a large number of uranium tailings have become a long potential for radioactive sources [14]. Uranium tailings contain many radionuclides, heavy metal poisons, and certain salts. Uranium tailings will seriously endanger the surrounding ecological environment and human health. Therefore, it is necessary to explore a low-cost, effective, and easy-to-use treatment of uranium tailings.

As early as 1973, Boquet et al. [15] found that soil bacteria induced calcite crystal deposition phenomenon; this phenomenon of microbial mineralization has aroused the concern of scholars at home and abroad. Researchers have found that carbonate mineralization plays an important role in the natural diagenesis process [16]. Minerals can be used to form mineral calcium carbonate crystals (composed of bacteria, cell secretions, and calcium carbonate) [17]. Microbes can cement the surrounding loose debris material into a hard rock through the role of an organic matrix [18, 19]. For example, carbonate mineralization can be used to carry out the sand body cementation test, according to researchers at the University of Murdoch in Australia [20]. In another study, van Paassen et al. [21] used microbial grouting reinforcement technology to study the mineralization cementation of bacteria and gravel. Based on the above inspiration, biocalcium carbonate cemented loose particles which can be used as a new and environmentally friendly cementing material have been paid tremendous attention by scholars at home and abroad [20, 22]. However, the microbial and mineral synergistic cementation sand bodies are rarely reported.

Based on the above-mentioned enlightenment, we have studied the morphology of mineralized crystals by controlling the mineralization time and concentrations of MMT. At the same time, we also investigated the effect of carbonate mineralized cementation uranium tailings by controlling the amount of MMT. It is expected to provide theoretical support and scientific basis for the application of biocalcium carbonate cementing technology in uranium tailings. Through the in situ cementing of biocalcium carbonate, it is necessary to avoid the shortcomings of high-energy, high-emission, and field construction in the traditional high-pressure grouting cementing process, which is higher for the equipment and the influence on the surrounding environment. At the same time, biocalcium carbonate cementing technology provides some reference for disposal of other tailings and radioactive solid waste.

2. Materials and Methods

2.1. Materials
2.1.1. Uranium Tailings

Uranium tailings are taken from Hengyang (Hunan). The radioactive was measured by Gamma Spectrometer System (ORTEC). The gamma radiation dose rate of uranium tailings is shown in Table 1.

The radioactive background level is in the range of 42.9–92.6 nGy/h, and the radioactivity of uranium tailings is in the range of 0.14120.2795 μGy/h.

2.1.2. Reagents

Reagents contain calcium nitrate (analytical grade, Chengdu Cologne Chemical Co., Ltd.), peptone (analytical grade, Sinopharm Group Pharmaceutical Co., Ltd.), NaCl (analytical grade, Chengdu Cologne Chemical Co., Ltd.), glucose (Analytical Pure, Sinopharm Group Pharmaceutical Co., Ltd.), anhydrous ethanol (analytical grade, Chengdu Cologne Chemical Co., Ltd.), and deionized water.

2.2. Methods
2.2.1. Strain Culture

Bacillus pasteurii was inoculated into beef extract-peptone agar medium (beef extract 10 g/L, peptone 5 g/L, sodium chloride 5 g/L, H2O 1 L, agar 15 g/L, and pH value 8.0). Bacillus pasteurii was inoculated into a liquid medium (glucose 20 g/L, peptone 10 g/L, sodium chloride 10 g/L, H2O 1 L, and pH 8.0). The bacteria were cultured in a biochemical incubator at 30°C for 24 h.

2.2.2. Mineralization Experiments

1 L of liquid medium was prepared using deionized water, glucose 20 g, peptone 10 g, and NaCl 5 g. And 0.05 mol/L (C1), 0.1 mol/L (C2), and 0.15 mol/L (C3) of Ca2+/urea through the microporous membrane filter sterilization method added to the above liquid medium. The liquid medium was incubated at 30°C for 12, 24, and 48 h, respectively. The mineralized sample was obtained by static and suction filtration, and the mineralized sample was washed three times with distilled water and absolute ethanol and then dried at 45°C for 24 h.

2.2.3. Preparation of Monolayer MMT Solution

10 g of MMT was dissolved in 1 L of deionized water and stirred at 400 rpm/min for one week. And then the solution was centrifuged at 3000 rpm for 10 min. The supernatant was the desired monolayer MMT solution.

2.2.4. Mineralization Experiment by Synergistic MMT

The liquid medium was added to 10 ml of sterilized monolayer MMT solution, and then 5 mL of bacterial liquid was inoculated, respectively. The liquid medium was incubated at 30°C for 12, 24, and 48 h, respectively. The mineralized sample was obtained by static and suction filtration, and the mineralized sample was washed three times with distilled water and absolute ethanol and then dried.

2.2.5. Mineralization Experiment by Adjusting the Temperature and pH

1 L of liquid medium was prepared using deionized water, glucose 20 g, peptone 10 g, and NaCl 5 g. And 0.1 mol/L of Ca2+/urea through the microporous membrane filter sterilization method added to the above liquid medium. pH was adjusted to 6.0, 7.0, 8.0, 9.0, and 10.0, respectively. The liquid medium was incubated at 30°C for 24 h.

1 L of liquid medium was prepared using deionized water, glucose 20 g, peptone 10 g, and NaCl 5 g. And 0.1 mol/L of Ca2+/urea through the microporous membrane filter sterilization method added to the above liquid medium. Temperature was adjusted to 10°C, 20°C, 30°C, 40°C, and 50°C, respectively. The liquid medium was incubated for 24 h.

2.2.6. Cementing of Uranium Tailings

The bottom of the 10 mL cylindrical plastic syringe out of the plug is filled with 1 cm thick medical gauze. The mixed homogeneous uranium tailings are injected into the syringe, the mass fraction of MMT in the mixed homogeneous uranium tailings was 0%, 2%, 4%, 6%, and 8%, and then the liquid medium was added to the syringe. The mineralization fluid was injected into the syringe after 12 h; after a certain period of time, the cemented sand column is made by stripping drying.

2.3. Characterization

The mineralized samples amplified 1000–20000 times were observed by the SEM. The crystal forms were analyzed by X-ray diffraction analyzer, Cu Kα radiation, wavelength 1.54056 Å, tube voltage 40.0 kV, tube current 100 mA, scan range 3°–80°, and scan step 0.02°. In the temperature range of 40–900°C, mineralized samples were characterized by TG at the heating rate of 20°C/min. XPS analyses were carried out with a Kratos Axis Ultra (Kratos Analytical, Manchester, UK) photoelectron spectrometer. The instrument uses a monochromatic Al Kα X-ray source. FT-IR spectra of mineralized samples were taken by Nicolet Avatar 370 of FT-IR spectrometer (Thermo Nicolet, USA) at wavenumbers from 4000 to 400 cm−1. The products mixed with KBr were compressed into tablet and analyzed by infrared spectroscopy, resolving power 4 cm−1, scanning 20 times, scanning range of 4000–400 cm−1.

3. Results and Discussion

3.1. MMT and Carbonate Mineralization Synergistically Form Mineralized Crystals

Figures 1(a)1(c) show the SEM images of mineralized samples by different time without MMT. It can be seen from Figures 1(a)1(c) that calcium carbonate crystals have different sizes of spherical or spherical aggregates, which slowly transformed into regular cuboids, cuboid aggregation crystals, or irregular aggregates with increasing mineralization time; the spherical calcium carbonate crystals disappeared substantially (Figure 1(c)). Figures 1(d)1(f) depict the SEM images of the mineralized sample by different time with MMT. At the beginning of mineralization, calcium carbonate crystals are basically spherical and spherical aggregates (Figures 1(d)1(f)). These spherical calcium carbonate crystals are formed by the accumulation of a piece of calcium carbonate film (Supporting Information, Figure S1, in Supplementary Material available online at https://doi.org/10.1155/2017/7874251). As the mineralization time increases, the spherical crystal slowly changes into a spindle, dumbbell, and irregular polyhedron-shape crystal (Figure 1(f)).

Figures 2(a)2(c) depict the SEM images of mineralized samples by different concentrations of Ca2+ without MMT. When the calcium ion concentration is 0.1 mol/L, the shape of the mineralized sample includes spherical, ellipsoidal, and plate type (Figure 2(b)). The shape of the mineralized samples has become almost irregular polyhedron, which was mixed with the part-spherical at 0.15 mol/L Ca2+ (Figure 2(c)). Figures 2(d)2(f) depict the SEM images of the mineralized sample by different concentrations of Ca2+ with MMT. The shape of the mineralized sample includes spherical and spherical aggregates with MMT at 0.05 mol/L Ca2+ (Figure 2(d)). It can be seen from the surface of the mineralized sample that calcium carbonate crystals are accumulated by a piece of calcium carbonate film (Supporting Information, Figure S1). With the increase of Ca2+ concentration, these spherical crystals transformed to almost square, spindle, and irregular polyhedron-shaped crystals (Figures 2(e) and 2(f)).

As can be seen from Figure 2(a), there are some holes with a mean diameter of 8 μm on the samples’ surface, which should be traced to death of microbe according to the size of the cell. It indicates that the microbe was involved in the formation of calcium carbonate crystals. By the SEM images, we can infer that MMT has a contribution in the regulation for calcium carbonate crystal form. At the same time, Ca2+ concentration played a certain regulating role on the morphology of calcium carbonate crystal.

Figure 3 shows the XRD patterns of calcium carbonate. According to the relevant data of PDF standard card (number 00-005-0586 and number 00-033-0268), with different Ca2+ concentration, the diffraction peak of calcite and vaterite appeared. Calcite’s main diffraction angle position 2θ of 23.0°, 29.4°, 35.9°, 39.5°, 43.1°, 47.5°, and 48.5° was approximately corresponding to (012), (104), (110), (113), (202), (018), and (116) crystal plane, respectively, and the vaterite diffraction angle position 2θ of 21.0°, 32.7°, 43.8°, and 49.0° was approximately corresponding to (002), (102), (110), and (104) crystal plane, respectively (Figure 3). It can be seen from Figures 3(a) and 3(c) that calcite and vaterite appear in the mineralized samples at different time under the condition of Ca2+ concentration of 0.1 mol/L, regardless of whether the MMT is added [23]. And the characteristic peaks of calcite continue to increase with time. The characteristic peaks of vaterite gradually weaken and disappear (Figures 3(a) and 3(c)). Finally, when Ca2+ concentration was 0.1 mol/L with 48 h, X-diffraction peak of the sample was only the one of calcite (Figures 3(a) and 3(c)) [24]. At the same mineralization time, the mineralized samples without MMT had only the characteristic peak of calcite (Figure 3(b)), while the mineralized samples with MMT coexisted with the vaterite (Figure 3(d)). However, with the increase of the concentration, the vaterite in the mineralized sample with MMT gradually disappeared, and the peak of the calcite gradually increased (Figure 3(d)). It can be seen that high Ca2+ concentration promotes the formation of calcite. All the analysis shows that MMT has a certain regulation effect on the crystal form of calcium carbonate. The XRD analysis results are consistent with the SEM morphologies.

Figure 4 illustrates the TGA data of calcium carbonate with and without MMT. Figure 4 showed that thermal decomposition behavior of calcium carbonate was similar. The whole decomposition process was accompanied by three weight loss stages [25]. The first one before 385°C was associated with the loss of crystal water. The second stage of the weight loss in the temperature range of 385–570°C corresponded to thermal decomposition of bacteria and their metabolites in the sample of the calcium carbonate. It was found that the weight loss of the mineralized samples with MMT (9.9%) was higher than that of the mineralized samples without MMT (3.4%). The reason may be that some of the material in the mineralized samples with MMT is decomposed. Between 570 and 800°C, a large thermal decomposition which was observed occurs, which could be attributed to the decomposition of calcium carbonate. In addition, the mineralized samples without MMT showed a more obvious weight loss than the mineralized samples with MMT.

It can be seen from Figure 4 that the weight loss rate of mineralized samples with MMT is larger than that without MMT. There are two reasons for the difference in weight loss. On the one hand, there are bits of MMT which contained some organic matter in the mineralized samples. And the decomposition of these organic substances at high temperatures leads to greater weight loss. It is found that the MMT has a large weight loss of about 200 to 500°C which is consistent with Xie’s experimental results [26]. On the other hand, it can be seen from Table 2 that the Ca2p electron binding energy shifted from 349.49 eV to 332.17 eV in the mineralized sample system with MMT. The more stable material would result from higher binding energy. The lower binding energy could lead to a greater weight loss for mineralized samples with MMT. From the above discussion, we can speculate that the MMT is involved in the crystallization process of calcium carbonate.

The FT-IR spectra of calcium carbonate prepared by different Ca2+ concentrations with different time with a wavelength range of 4000–400 cm−1 are shown in Figure 5. FT-IR absorption peaks include the characteristic peaks of calcite at 870.7/710.4 cm−1. Moreover, the peaks at 1085.7 cm−1 belong to the vaterite peaks (Figure 5(a)), which was basically consistent with the description of the literature [27]. (Figure 5(a)), which is also in accordance with the description of literature [27]. The broad absorption peak of 3424.8 cm−1 is assigned to -O-H bond symmetric stretching vibration and asymmetric stretching vibration due to the existence of hydroxyl and adsorbed water on the surface of CaCO3 particles [28]. The peaks at 870.7/710.2 cm−1 are in-plane bending vibration and the out-plane bending vibration of [29]. In addition, the peak at 1425.1 cm−1 is the asymmetric stretching vibration of C=O.

As can be seen from Figure 5(a), the absorption peaks of and C=O are increasing with the increase in mineralization time, which can show that the crystal form of calcium carbonate is changing, which is consistent with the previous XRD analysis. From Figure 5(b) it can be obtained that the characteristic peaks of the spectrum are almost the same in Ca2+ concentration of 0.05, 0.1, and 0.15 mol/L. And in Ca2+ concentration of 0.15 mol/L, the characteristic peaks were significantly higher than other concentrations. It indicates that Ca2+ concentration has a certain regulation effect on the crystal form of calcium carbonate. Figures 5(c) and 5(d) are the infrared spectra of the mineralized samples with the MMT. Compared with Figures 5(a) and 5(b), the position of the characteristic peaks shifted and the intensity of the characteristic peaks weakened. It can be seen from Figure 5(d) that when the concentration of calcium ions is 0.15 mol/L, the intensity of the characteristic peaks reaches a maximum value. We conclude that MMT played a certain regulatory role in the microbial mineralization of calcium carbonate crystals.

Table 2 lists the inner electron binding energy data for mineralized samples with MMT and without MMT. From the X-ray photoelectron spectroscopy (XPS) of O1s (Figures 6(a) and 6(b)), it was found the O1s electron binding energy was 522.87 eV in the XPS spectrum without MMT, but in the MMT system, it reduced to 520.62 eV. And the Ca2p electron binding energy shifted from 349.49 eV to 332.17 eV in the mineralized sample system with MMT, and the peak intensity was changed greatly. It can be explained by the fact that MMT and Ca2+ played a certain regulatory role in the microbial mineralization of calcium carbonate crystals, which is consistent with the redshift of the characteristic peaks of .

The chemical shift of the X-ray photoelectron spectroscopy is a reflection of the change in the electronic environment surrounding the electron binding energy of the inner layer. In the formation of complexes, the nonbond orbital of the donor undergoes electron charge transfer to the unoccupied orbital of the acceptor, which inevitably exhibits an increase in the electron binding energy of the donor atoms and a decrease in the electron binding energy of the acceptor atoms. It can be seen that the O1s electron binding energy (2.25 eV) in the C=O group is lower than mineralized sample system with MMT, and the coordination of O1s and Ca2p leads to the change of O1s’ electron binding energy. At the same time, the electron binding energy of Ca2p changed greatly, which decreased by 17.32 eV. This seems to be a reflection of the greater impact of MMT on the filling of Ca2p electrons by subfitting of O1s and Ca2p, respectively. In the mineralized sample system without MMT, O1s mainly existed in two chemical states (Figure 6(c)), and the binding energies of O1s were 531.8 eV and 532.8 eV, respectively. Ca2p also had two chemical states (Figure 6(e)), and the binding energies of Ca2p were 347.5 eV and 350.8 eV. In the mineralized sample system with MMT, the binding energies of O1s were changed to 531.1 eV and 532.0 eV (Figure 6(d)), and the binding energies of Ca2p were 346.6 eV and 350.5 eV (Figure 6(e)), respectively. This is probably due to the fact that MMT and calcium ions have a chemical effect. Furthermore, the MMT involved in microbial mineralization deposition of calcium carbonate process can also explain this behavior.

3.2. Effects of Temperature and pH on Microbial Mineralization

In order to efficiently cement the uranium tailings, we studied the effect of temperature and pH on microbial mineralization at different temperatures and pH conditions by varying the number of bacteria and the amount of calcium carbonate produced in the mineralized solution. As can be seen from Figure 7(a), we can see that the number of bacteria in the mineralized solution firstly increased and then decreased, and the number of bacteria reached the maximum at pH = 8 with the increase of pH value. Figure 7(b) shows that the number of bacteria in the mineralized solution increases first and then decreases, and the number of bacteria reaches the maximum at about 30°C with the temperature increases. Figures 7(c) and 7(d) show the effect of temperature and pH on the amount of calcium carbonate produced by microorganisms. It can be seen from Figure 7(c) that the amount of calcium carbonate reaches a maximum at pH = 8. It can be seen from Figure 7(d) that there is no calcium carbonate precipitation at 0 and 50°C, and it reaches the maximum at about 30°C. In addition, the amount of bacteria in the mineralization solution of montmorillonite was significantly higher than the control group, and the amount of calcium carbonate produced was significantly larger than the control group. So we can conclude that MMT for microbial mineralization process has a certain role in promoting. From the above analysis, it can be concluded that the activity of bacteria is the best at a temperature of 30°C and the pH value is 8. The above conclusions can provide the best conditions for the subsequent microbial mineralization of uranium tailings.

3.3. The Mechanism of MMT Fixed Microbial Solidification of Uranium Tailings

Microbial-induced calcium carbonate mineralization deposition is considered to be a more complex process than chemical action to induce calcium carbonate deposition. The surface of the microbial cell contains many ions which are capable of inducing mineral precipitation by providing a nucleation site [30]. As shown in Figure 8, microbes can produce a large number of urea hydrolases with high activity in their own metabolic activity. They can use urea as a nutrient source in the culture medium, produce ammonia, and carbon dioxide, so that the solution of concentration is increased. Resulting in a slight increase in the pH value of the solution, more conducive to the growth and reproduction of the cells, the role of enzyme has also been continuously enhanced. At the same time, the negatively charged water-soluble organic matter at the interface of the cell membrane began to chelate Ca2+, and the concentration of local crystal anions () was further increased, microorganism itself as a nucleation site to form calcium carbonate crystals when the concentration of amorphous calcium carbonate precursors reaches saturation. Due to the fact that montmorillonite itself shows negative charge, MMT can be chelated with calcium ions to form chelated calcium ions, chelating calcium ions interact with microorganisms to form calcium carbonate mineral crystals [27]. What is more, MMT can balance the acidity and ions in the uranium tailings (Supporting Information, Figure S2), and it also can reduce the toxicity of uranium ions on microorganisms (Supporting Information, Figure S3) [31]. In addition, MMT filling in the gap between the uranium tailings made the cement body more stable. With the increase of mineralization time, calcium carbonate crystals fill the gap between the uranium tailings, the uranium tailings particles tightly stick together, so that the uranium tail slags into a high strength cement pillar [32].

3.4. 3.4 Microbial Mineralization of Uranium Tailings

As can be seen from Figure 9(a), uranium tailings migrated from the loose particles into certain strength of the cement pillar with the mineralization time increased. Figure 9(b) is the cemented sand column by adding different amounts of MMT. Figures 9(c)9(f) depict the SEM images of the cemented sand column.

Figures 9(d)9(f) are a cemented sand column by adding MMT. Compared with the cemented column without MMT, the uranium tailings in cemented sand column were combined much tighter because of the presence of MMT (Figure 9(c)). In addition, there are dense calcium carbonate crystals which are formed at the gap among the uranium tailings in cemented sand column. As can be seen from Figures 9(c)9(f), uranium tailings would be cemented sand column whether MMT was added, but MMT can make cement pillar more compact.

The XRD patterns in Figure 10 show that the composition of cemented sand column contains not only SiO2 but also obvious CaCO3 peak, where calcite and vaterite are present at (104), (113), (202), and (118). With the increase of the amount of MMT, the characteristic peak of calcite gradually increased. We speculate that MMT may be carried out on the retention of radionuclides by good adsorption and ion exchange; thereby it can reduce the toxicity of radionuclides to bacteria.

It can be seen from Figure 11 that with the increase of the amount of MMT, the compressive strength of cemented sand column increases. And when the content of MMT is 6%, the compressive strength reaches the maximum value, the compressive strength of 2.18 MPa, which increased by 47.66% compared with the sample without MMT. The compressive strength of the cement body gradually decreased due to the calcium carbonate crystal which could not penetrate inward with the increase of the amount of MMT, which showed that calcium carbonate crystals form a dense calcium carbonate film on the periphery of the cemented body, thereby preventing the cementation of the internal uranium tailings.

4. Conclusions

Based on microbial mineralization cementation of uranium tailings, the results from SEM, FT-IR, XRD, TGA, and XPS and the compression tests indicated that MMT and microbes have some kinds of interaction, to a certain extent, which can regulate the crystal form of calcium carbonate. In addition, MMT negatively charged can be combined with calcium ions to form chelated calcium ions, promoting the formation of calcium carbonate crystals. In the process of microbial mineralization cementation of uranium tailings, MMT can balance the pH and ions in the uranium tailings; it also can reduce the toxicity of uranium ions on microorganisms. The compressive strength of the cemented sand column with MMT was significantly improved by comparison with the cemented sand column without adding MMT, which indicated that the MMT played a certain role in the microbial mineralization of the uranium tailings. As can be seen from the above it is reasonable and feasible to induce the deposition of biocalcium carbonate cemented uranium tailings by carbonate mineralization microbe. The analysis results can provide the basis for the theoretical research and practical application of uranium tailings.

Conflicts of Interest

The authors declare that they have no conflicts of interest.

Acknowledgments

This work was financially supported by Fundamental Science on Nuclear Wastes and Environmental Safety Laboratory (nos. 14zxnk02 and 15kffk02), Sichuan Province Science and Technology Pillar Program (no. 2014GZ0185), National Natural Science Foundation of China (nos. 21601147 and 21406182), Key Research and Development Project of Science and Technology Department of Sichuan Province (no. 2017GZ0342), Projects in the Sichuan Province Science and Technology Pillar Program (nos. 2016GZ0259 and 2016GZ0277), and Plan Projects of Mianyang Science and Technology (nos. 15zd2110 and 15zd2102). The authors acknowledge the Southwest University of Science and Technology Analysis and Testing Center for the help of sample testing.

Supplementary Materials

Figure S1: Typical SEM images of mineralized samples of montmorillonite.

Figure S2: Change of pH in the mineralization solution.

Figure S3: Effects of different additives on bacterial growth.

  1. Supplementary Material