The effect of transferrin (Tf) on iron uptake and ROS by cultured erythroid precursors. Cultured human erythroid precursors were harvested on days 7-8 of phase II, washed and loaded with CA or DCF. (a) CA-loaded cells were washed and resuspended in PBS or in phase II medium (complete medium, containing 30% serum), and incubated for 1- or 2 hrs with the indicated concentrations of FAS. (b) DCF-loaded cells were washed and resuspended in PBS, and incubated for 1- or 2 hrs with the indicated concentrations of FAS. (c) CA-loaded cells were washed and resuspended in PBS supplemented with or without human holo-Tf (300 μg/mL) and incubated for 1–3 hrs with the indicated concentrations of FAS. The results indicated reduced iron uptake in the presence of Tf. The results are expressed as the percent (%) change (mean ± SD, ) in the MFIs following incubation with and without Fe, calculated per the MFI−Fe.
