Research Article

Sickle Cell Disease Activates Peripheral Blood Mononuclear Cells to Induce Cathepsins K and V Activity in Endothelial Cells

Figure 2

TNFα and PBMC interactions increase JNK and Akt phosphorylation. Confluent HAECs were cocultured with peripheral blood mononuclear cells isolated from AA or SS donors, and lysates were collected for kinase analysis. Levels of phosphorylated (a) JNK, (b) c-Jun, (c) Akt, and (d) ERK1/2 were measured, and phosphorylated kinase signals were normalized to unstimulated HAEC control ( , , SEM bars shown).