FK228 Analogues Induce Fetal Hemoglobin in Human Erythroid Progenitors
Figure 3
JMA33 and JMA26 induce HbF in primary erythroid cells. (a) Day 11 erythroid progenitors were treated for 48 hr with the controls agents and the FK228 analogues shown and then analyzed by RT-qPCR. Untreated cells (UT) were used as a control and normalized to one. Data were calculated as the means ± SEM. (b) Progenitors were stained with anti-γ-globin FITC conjugated antibody overnight and HbF positive cells for were visualized. DAPI staining was performed to identify cell nuclei and to determine cell counts. Images were photographed at 40X power. The images generated were used to count 500 DAPI positive cells and the %FITC positive cells were calculated accordingly.
