Anemia

Research Article

FK228 Analogues Induce Fetal Hemoglobin in Human Erythroid Progenitors

Table 1

γ-globin induction in KU812-γF/βR stable lines by FK228 derivatives¹.


Line 1	Drug concentration	Mean	SEM	value	Fold change

Untreated	none	0.0402	0.0091	n/a	1
Cys	10 mM	0.0388	0.0092	0.8528	0.950
Hemin	50 μM	0.1732	0.0399	0.0050	4.325
SAHA	2000 nM	0.2157	0.0142	0.0001	5.400
FK228	1.5 nM	0.1197	0.0325	0.0150	2.975
1127Ox	1000 nM	0.0363	0.0022	0.8167	0.900
JMA1	1.5 nM	0.0473	0.0149	0.6720	1.175
JMA2	1.0 nM	0.0157	0.0003	0.1629	0.400
JMA12	1000 nM	0.0187	0.0009	0.2156	0.475
JMA26	500 nM	0.1460	0.0234	0.0004	3.650
JMA33	1000 nM	0.1373	0.0117	0.0002	3.425
JMA112	100 nM	0.0187	0.0007	0.2156	2.453

Line 2	Drug concentration	Mean	SEM	value	Fold change

Untreated	none	0.1573	0.0150	n/a	1
Cys	10 mM	0.1200	0.0056	0.1977	0.764
Hemin	50 μM	0.6593	0.0839	0.0001	4.261
SAHA	2000 nM	0.6500	0.0208	0.0001	4.140
FK228	1.5 nM	0.6167	0.0338	0.0001	3.929
1127Ox	1000 nM	0.6133	0.0371	0.0001	3.904
JMA1	1.5 nM	0.1260	0.0152	0.2896	0.803
JMA2	1.0 nM	0.1183	0.0071	0.1814	0.752
JMA12	1000 nM	0.1470	0.0027	0.7093	0.936
JMA26	500 nM	0.2487	0.0308	0.0153	1.579
JMA33	1000 nM	0.3313	0.0256	0.0002	2.108
JMA112	100 nM	0.1070	0.0076	0.0938	0.682

Line 3	Drug concentration	Mean	SEM	value	Fold change

Untreated	none	0.0721	0.0052	n/a	1
Cys	10 mM	0.0860	0.0050	0.1814	1.194
Hemin	50 μM	0.2508	0.0153	0.0001	3.486
SAHA	2000 nM	0.2520	0.0066	0.0001	3.500
FK228	1.5 nM	0.1583	0.0198	0.0007	2.194
1127Ox	1000 nM	0.0370	0.0095	0.0076	0.514
JMA1	1.5 nM	0.0770	0.0036	0.6179	1.069
JMA2	1.0 nM	0.0740	0.0036	0.8463	1.027
JMA12	1000 nM	0.0537	0.0023	0.0779	0.750
JMA26	500 nM	0.2560	0.0192	0.0001	3.555
JMA33	1000 nM	0.5120	0.1765	0.0007	7.111
JMA112	100 nM	0.0467	0.0019	0.0216	0.653

¹Using three independent KU812-γF/βR stable cell lines, the FK228 analogues were examined for their ability to induce γ-globin. After 48 hr drug treatments, cells were harvested and dual luciferase assay performed. Untreated cells were used as a control and normalized to one. Data were calculated as the means standard error of the mean (SEM).