Correlation between Plasma Interleukin-3, the α/β Globin Ratio, and Globin mRNA Stability

Table 2

Determination of the real-time PCR efficiencies.

	Concen.	Log	Mean Ct	Ct1	Ct2	Ct3	Slope	Effic. %
	50	1.69897	16.32	16.32	16.31	16.33	−3.30089	101
	25	1.39794	17.22	17.21	17.23	17.22
	12.5	1.09691	18.17667	18.22	18.15	18.16
	6.25	0.79588	19.31333	19.27	19.31	19.36
	50	1.69897	16.48667	16.46	16.47	16.53	−3.24109	103
	25	1.39794	17.29	17.28	17.3	17.29
	12.5	1.09691	18.27667	18.32	18.17	18.34
	6.25	0.79588	19.41	19.45	19.36	19.42
	100	2	24.76667	24.54	24.92	24.84	−3.61426	89
	50	1.69897	25.85	25.82	25.95	25.78
	25	1.39794	26.91	26.94	26.88	26.91
	12.5	1.09691	28.04333	28.05	28.05	28.03
	6.25	0.79588	29.11	29.02	29.14	29.17
GAPDH	100	2	31.395	31.3		31.49	−3.65744	88
	50	1.69897	32.11	32.06		32.16
	25	1.39794	33.775	33.52	34.03
	12.5	1.09691	34.51		34.85	34.17