Research Article
Correlation between Plasma Interleukin-3, the α/β Globin Ratio, and Globin mRNA Stability
Table 2
Determination of the real-time PCR efficiencies.
| | Concen. | Log | Mean Ct | Ct1 | Ct2 | Ct3 | Slope | Effic. % |
| | 50 | 1.69897 | 16.32 | 16.32 | 16.31 | 16.33 | −3.30089 | 101 | 25 | 1.39794 | 17.22 | 17.21 | 17.23 | 17.22 | | 12.5 | 1.09691 | 18.17667 | 18.22 | 18.15 | 18.16 | | 6.25 | 0.79588 | 19.31333 | 19.27 | 19.31 | 19.36 | |
| | 50 | 1.69897 | 16.48667 | 16.46 | 16.47 | 16.53 | −3.24109 | 103 | 25 | 1.39794 | 17.29 | 17.28 | 17.3 | 17.29 | | 12.5 | 1.09691 | 18.27667 | 18.32 | 18.17 | 18.34 | | 6.25 | 0.79588 | 19.41 | 19.45 | 19.36 | 19.42 | |
| | 100 | 2 | 24.76667 | 24.54 | 24.92 | 24.84 | −3.61426 | 89 | 50 | 1.69897 | 25.85 | 25.82 | 25.95 | 25.78 | | 25 | 1.39794 | 26.91 | 26.94 | 26.88 | 26.91 | | 12.5 | 1.09691 | 28.04333 | 28.05 | 28.05 | 28.03 | | 6.25 | 0.79588 | 29.11 | 29.02 | 29.14 | 29.17 | |
| GAPDH | 100 | 2 | 31.395 | 31.3 | | 31.49 | −3.65744 | 88 | 50 | 1.69897 | 32.11 | 32.06 | | 32.16 | | 25 | 1.39794 | 33.775 | 33.52 | 34.03 | | | 12.5 | 1.09691 | 34.51 | | 34.85 | 34.17 | |
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