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Anemia
Volume 2015 (2015), Article ID 848921, 6 pages
http://dx.doi.org/10.1155/2015/848921
Research Article

Hyperglycaemic Environment: Contribution to the Anaemia Associated with Diabetes Mellitus in Rats Experimentally Induced with Alloxan

Department of Biomedical Sciences, Faculty of Basic Medical Sciences, College of Health Sciences, Ladoke Akintola University of Technology, Ogbomosho 210214, Oyo State, Nigeria

Received 16 September 2015; Revised 10 November 2015; Accepted 12 November 2015

Academic Editor: Aurelio Maggio

Copyright © 2015 Oseni Bashiru Shola and Fakoya Olatunde Olugbenga. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Diabetes mellitus characterized by hyperglycaemia presents with various complications amongst which anaemia is common particularly in those with overt nephropathy or renal impairment. The present study has examined the contribution of the hyperglycaemic environment in diabetic rats to the anaemia associated with diabetes mellitus. Method. Sixty male albino rats weighing 175–250 g were selected for this study and divided equally into control and test groups. Hyperglycaemia was induced with 170 kgbwt−1 alloxan intraperitoneally in the test group while control group received sterile normal saline. Blood samples obtained from the control and test rats were assayed for packed cell volume (PCV), haemoglobin (Hb), red blood cell count (RBC), reticulocyte count, glucose, plasma haemoglobin, potassium, and bilirubin. Result. Significant reduction () in PCV ( versus ) and haemoglobin ( versus ) with significant increase () in reticulocyte count ( versus ), plasma haemoglobin ( versus ), and potassium ( versus ) was obtained in the test while plasma bilirubin showed nonsignificant increase ( versus ). Conclusion. The increased plasma haemoglobin and potassium levels indicate an intravascular haemolytic event while the nonsignificant increased bilirubin showed extravascular haemolysis. These play contributory roles in the anaemia associated with diabetes mellitus.